不同灸法对类风湿性关节炎家兔肿胀关节及滑膜液中白介素-1、肿瘤坏死因子-α表达的影响  

Effect of Different Types of Moxibustion Intervention on Expression of Inflammatory Cytokines IL-1 and TNF-α in Rabbits with Rheumatoid Arthritis

作　　者：杨涵棋[1] 刘旭光[1] 杨馨[1] 陈婷[1] 余曙光[1] 

机构地区：[1]成都中医药大学针灸推拿学院,成都610075

出　　处：《针刺研究》2013年第2期134-139,共6页Acupuncture Research

基　　金：国家自然科学基金课题(No.81173319);国家重点基础研究发展计划课题(2009CB 522903)

摘　　要：目的:观察不同灸法对实验性类风湿性关节炎(RA)家兔关节滑膜液中白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)表达的影响,探讨不同灸法治疗实验性RA的作用机制。方法:日本大耳白兔40只,随机分为对照组、模型组、直接灸组、隔姜灸组及温和灸组,每组8只。采用弗氏完全佐剂法复制实验性RA模型,于造模后第7天开始,分别用直接灸、隔姜灸、温和灸施灸"肾俞""足三里"穴各5壮,两侧交替进行,每日1次,6d为1个疗程,共3个疗程。在造模前、造模后第7天及治疗第7、14、21天分别测量各组后肢的左、右膝关节周长;用酶联免疫法检测滑膜液中IL-1、TNF-α的含量。结果:直接灸、隔姜灸、温和灸均能明显降低RA白兔左、右膝关节周长(P<0.01),其中以隔姜灸作用最为明显(P<0.05);直接灸、隔姜灸、温和灸均能明显降低RA模型动物关节滑膜液中IL-1、TNF-α含量(P<0.01,P<0.05),以隔姜灸作用最为明显(P<0.05)。结论:不同灸法治疗实验性RA均有明显的抗炎效应,且以隔姜灸作用最为显著;不同灸法抑制IL-1、TNF-α炎性反应因子表达可能是其发挥抗炎效应的重要机制之一。Objective To observe the effect of different moxibustion intervention on expression of interleukin-1 （IL-1） and tumor necrosis factor-α （TNF-α） in the synovial fluid of hind-knee joint in rheumatoid arthritis （RA） rabbits. Methods Forty Japanese big-ear white rabbits （half male and half female） were randomized into normal control, RA model, direct-moxibustion, ginger-partitioned moxibustion and warm moxibustion groups （n = 8）. RA model was established by injection of Freund＇s Com- plete Adjuvant （0.5 mL/kg） into the articular cavities of the rabbits＇ bilateral hind-limbs. Moxibustion intervention was applied to unilateral ＂Shenshu＂ （BL 23） and ＂Zusanli＂（ST 36） regions alternatively for 20 min from the 7thday on after modeling, once daily for 3 weeks except Sundays. The circumference of the hindlimb-knee joint was measured using a tape measure and the contents of IL-1 and TNF-α in the synovial fluid of articular cavities were detected by ELISA. Results In comparison with the normal control group, the circumference values of the bilateral hind-knee joints, and the contents of IL-1 and TNF-α in the synovial fluid of articu- lar cavities in the model group were significantly increased （P^0.01 ）. After the moxibustion treatment, compared with the model group, the circumference values of the bilateral hind-knee joints, and IL-1 and TNF-α contents of the synovial fluid in the warm moxibustion, direct moxibustion and ginger-partitioned moxibustion groups were remarkably reduced （P〈0.01, P〈0.05）. The effects of the ginger-partitioned group were significantly superior to those of both warm moxibustion and direct moxibustion groups in decreasing the swelled hind-knee joint circumference on day 21 after the treatment and down-regulating synovial fluid inflamma- tory cytokines IL-1 and TNF-α levels （P〈0.05）. Conclusion Warm, direct and ginger-separated moxibustion interventions all can reduce inflammatory reactions of the knee-joint and suppress inflammatory

关 键 词：类风湿性关节炎 隔姜灸 直接灸 温和灸 炎性反应 滑膜液IL-1 TNF-α 

分 类 号：R593.22[医药卫生—内科学]