Wnt7a基因对大鼠骨髓间充质干细胞增殖及向神经元样细胞分化的影响  

Wnt7a gene stimulates mesenchymal stem cell proliferation and differentiation into neuron-like cells

作　　者：周长立[1] 任先军[1] 蒋涛[1] 王开见[1] 阴洪[1] 

机构地区：[1]第三军医大学新桥医院骨科,重庆400037

出　　处：《第三军医大学学报》2013年第8期702-706,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(81271362);国家自然科学基金青年科学基金(31000438);全军医学科技"十二五"科研重点项目(BWS11C040)~~

摘　　要：目的研究Wnt7a基因过表达对大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)增殖和向神经元样细胞分化的影响。方法构建Wnt7a腺病毒,利用病毒感染MSCs,MTT法检测Wnt7a基因腺病毒感染前后MSCs增殖情况,Western blot检测细胞质和细胞核中β-catenin及CyclinD1表达的变化,比较诱导后向神经元样细胞的分化率。结果与对照组细胞相比,Wnt7a腺病毒感染组细胞增殖能力明显增强(P<0.05);细胞质和细胞核中β-catenin蛋白表达量均明显增加(P<0.05);CyclinD1的蛋白表达量也明显增加(P<0.05);诱导后向神经元样细胞分化率明显提高。结论 Wnt7a蛋白表达上调,可能通过提高β-catenin和CyclinD1表达来促进MSCs细胞增殖,Wnt7a蛋白同时促进MSCs向神经元样细胞的诱导分化。Objective To explore the effect of up-regulation of Wnt7a on mesenchymal stem cells（MSCs） proliferation and differentiation into neuron-like cells.Methods An adenovirus vector expressing Wnt7a protein was constructed,and then MSCs were transfected with the recombinant vector.MSCs proliferation was detected by MTT assay,and the protein expression of cytoplasmic β-catenin,nuclear β-catenin and CyclinD1 was detected by Western blotting.The rates of MSCs differentiating into neuron-like cells were compared after transfection.Results Compared with the control MSCs,the MSCs transfected with Wnt7a recombinant adenovirus vector exhibited an increase in cell proliferation,and the protein expression of cytoplasmic β-catenin,nuclear β-catenin and CyclinD1 significantly increased（P0.05）.The rate of neuron-like cells in the MSCs transfected with Wnt7a recombinant adenovirus vectors was significantly higher than that in the control group and the non-transfection group（P0.05）.Conclusion Overexpression of Wnt7a protein promotes MSCs proliferation through up-regulating β-catenin and CyclinD1 expression,and stimulates MSCs differentiation into neuron-like cells,which provides further foundation for the therapy for spine cord injury.

关 键 词：腺病毒载体 骨髓间充质干细胞 Wnt7a基因 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R394-33[医药卫生—基础医学]