姜黄素对大鼠重症胰腺炎的干预效应研究  

Effects of curcumin intervention on chemokine ENA-78 expression in rat severe acute pancreatitis

作　　者：李晓萍[1] 张志坚 屈纪富[1] 

机构地区：[1]第三军医大学西南医院急救部,重庆400038 [2]巴南区人民医院ICU,重庆401320

出　　处：《第三军医大学学报》2013年第8期759-763,共5页Journal of Third Military Medical University

基　　金：全军医学科技"十二五"科研重点课题(BWS11J038);第三军医大学西南医院院管课题(2008XG19)~~

摘　　要：目的探讨趋化因子ENA-78(epithelial neutrophil activating peptide 78,ENA-78)在重症急性胰腺炎(acutepancreatitis,SAP)发病过程中的作用及姜黄素(curcumin,CUR)的干预效应。方法 54只SD大鼠按完全随机法分为SAP组、姜黄素治疗(SAP+CUR)组和对照组,用4%牛磺胆酸钠逆行胆胰管注射建立大鼠模型,SAP+CUR组在建立模型前2 h腹腔内注射姜黄素溶液(200 mg/kg),余组注射同等剂量的DMSO溶液。用ELISA法测定3组大鼠不同时点血清中ENA-78水平的变化;Western blot和免疫组化法检测胰腺组织不同时点ENA-78蛋白的表达水平;病理组织切片检查各时点胰腺的病理变化。结果 SAP组血清中ENA-78水平随时间的延长逐渐增强,3 h后各时点血清ENA-78水平均高于对照组及SAP+CUR组(P<0.01);SAP组及SAP+CUR组术后胰腺组织ENA-78蛋白表达随时间延长逐渐增加,术后各时间点表达均显著高于对照组,但同时间点比较SAP+CUR组表达明显低于SAP组(P<0.01);病理组织切片检查显示SAP+CUR组各时点与SAP组相比较,胰腺病理损伤显著减轻。结论姜黄素能有效干预SAP,其机制可能与抑制趋化因子ENA-78的表达有关。Objective To investigate the role of epithelial neutrophil activating peptide 78（ENA-78） in the pathogenesis of severe acute pancreatitis（SAP） and effects of curcumin intervention on ENA-78 expression in SAP.Methods Fifty-four SD rats were randomized into three groups： a SAP group,a curcumin treatment group（SAP＋CUR） and a control group.SAP model was induced by retrograde infusion of 4% sodium taurocholate into the bili-pancreatic duct in rats.Rats in the SAP＋CUR group were injected with curcumin（200 mg/kg） through abdominal cavity before operation while those in the control and SAP groups were injected with DMSO.ENA-78 in the serum was detected by ELISA at various time points.The expression of ENA-78 in pancreatic tissues was detected by Western blotting and immunohistochemistry.The histopathological changes were observed at the same time.Results Serum level of ENA-78 in 3 h after operation in the SAP group was significantly higher than that in the control group and SAP＋CUR group（P0.01）.Compared with the control group,the expression of ENA-78 in the pancreatic tissues of rats in the SAP group and SAP＋CUR group increased significantly in a time-dependent manner.Compared with the SAP group,the expression of ENA-78 in the pancreatic tissues of rats in the SAP＋CUR group was decreased（P0.01）.Pathological injury of pancreatic tissues was more obvious in the SAP group than in the SAP＋CUR group.Conclusion Curcumin efficiently interferes SAP and may be related to inhibition of ENA-78 expression.

关 键 词：重症急性胰腺炎 姜黄素 趋化因子ENA-78 基因表达 

分 类 号：R282.71[医药卫生—中药学] R576[医药卫生—中医学]