早熟凝集染色体环法在山西太原事故受照射者生物剂量估算中的应用  

Biological dose estimation by prematurely condensed chromosomes ring assay for five victims in Shanxi Taiyuan radiation accident

作　　者：姚波[1] 李玉芳[1] 白娟[1] 周振山[1] 王军良[1] 满秋红[1] 邱丽娟[1] 刘广贤[1] 郭梅[1] 艾辉胜[1] 

机构地区：[1]解放军第307医院血液科,全军放射病救治研究所,北京100071

出　　处：《癌变．畸变．突变》2013年第2期91-95,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨药物诱导的早熟凝集染色体环(prematurely condensed chromosomes ring,PCC-R)分析方法在辐射事故受照射者生物剂量估算中的应用。方法:山西太原辐射事故发生后16h收集5名受照射者(1、2、3、4和5号)的外周血,及1号受照者照射后23h的骨髓细胞和24h的外周血,采用冈田酸(Okadaic acid,OA)诱导的PCC方法计数PCC细胞中的PCC-R,以新建立的PCC-R剂量-效应曲线(1～20Gy)估算生物剂量。照射后31d对4名受照射者(2～5号)的PCC-R频率进行了分析。并采用常规染色体双加环(dic+r)、胞质分裂阻滞微核(cytokinesis-block micronuclei,CBMN)分析及物理方法对PCC-R法的结果进行验证。结果:最严重受照射者1号的外周血培养仅获得极少量的PCC细胞,而骨髓培养获得了一定数量的PCC细胞。以骨髓细胞PCC-R频率对1号及以外周血PCC-R频率对2～5号进行剂量估算的结果分别为12.4、3.6、3.2、1.7和1.5Gy。2～5号受照者照后31d PCC-R频率与照后16h相比下降幅度分别为51%、69%、69%及44%。结果与用dic+r、CBMN分析及物理方法估算的剂量接近,与临床表现基本相符。结论:经实际应用证明,药物诱导的PCC-R法简便、快速,新建立的PCC-R剂量-效应曲线准确、可靠。该法更适用于较高剂量照射的生物剂量估算,且应在照后尽早取血,最好在1个月之内。OBJECTIVE： To investigate the value of prematurely drug induced condensed chromosomes ring （PCC-R） assay in estimating the biological doses in the victims of radiation accident. METHODS ： Samples of peripheral blood were collected from the five victims （subjects 1-5） of the radiation accident of Taiyuan, Shanxi, 16 hours after the accident. Bone marrow samples were collected 23 h after and peripheral blood sample 24 h after the accident from subject 1. The frequencies of PCC-R in PCC cells obtained by Okadaic acid （OA） induction were counted. A biological dose estimation was performed by a dose-effect curve of PCC-R （1-20 Gy）. The frequencies of the PCC-R for the 4 victims （subjects 2-5） were analyzed 31 days after the accident. The results from PCC-R assay were verified by dicentrics plus centric rings （dic＋r）, Cytokinesis-block micronuclei （CBMN） assay and physical method. RESULTS： No or rare PCC cells were observed in the peripheral blood cultures from subject 1. However, PCC cells were obtained from bone marrow cultures in subject 1 and in peripheral blood cultures from subject 2-5. The doses estimated for subject 1-5 were 12.4, 3.6, 3.2, 1.7 and 1.5 Gy, respectively. A decrease of PCC-R frequencies were observed 31 days after the accident, and the extent of the decrease were 51%, 69%, 69% and 44% for the subjects 2-5, respectively, compared with the data 16 h after the accident. The estimated doses were in accordance with those doses estimated by dic＋r, CBMN assay, physical method and clinical symptoms. CONCLUSION： Drug induced PCC-R assay is convenient and rapid, and the new curve of PCC-R is accurate and reliable. It is especially suitable for estimating higher dose of irradiation. However, blood samples should be collected as soon as possible and should be done within one month after theaccident.

关 键 词：辐射事故 早熟凝集染色体环 生物剂量估算 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]