钽涂层人工假体植入早期假体表面骨长入特点及其骨组织相容性的初步研究  

Characteristics of bone-ingrowth on the surface of tantalum （Ta）-coated prosthesis after implantation

作　　者：段永宏[1] 朱庆生[1] 郭飞 李矛 蒋驰[4] 朱锦宇[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所骨关节科,西安710032 [2]解放军第一疗养院四疗区骨外科 [3]解放军第三○六医院骨科 [4]四川材料与工艺研究所

出　　处：《中华创伤骨科杂志》2013年第4期326-330,共5页Chinese Journal of Orthopaedic Trauma

基　　金：军队医药卫生科研项目（CWS11J151）;陕西省科技计划项目（2010K13-02-04）

摘　　要：目的初步探讨钽涂层假体表面骨长入特点及其骨组织相容性。方法将60只雄性新西兰兔随机分为钽涂层组和钛涂层组（n=30）、无菌条件下分别进行钽涂层假体与钛涂层假体股骨髁内植入术。分别于术后4、8、16周处死两组动物各10只，采用扫描电镜观察、能量色散X射线光潜仪（EDX）分析（分别取2个标本）、组织病理学观察（分别取8个标本）及骨组织形态计量学分析等方法对两组涂层假体表面附着组织的结构进行分析和量化。结果 假体植入后4周，假体表面扫描电镜观察及EDX分析发现两组涂层假体表面均有骨组织附着，新生组织小梁结构清晰。组织病理学观察显示假体植入后两组涂层与周围新生骨组织结合紧密，涂层稳定无脱落，假体周围的骨量随假体植入时间逐渐增加。骨形态计量学分析结果显示：术后4、8、16周钽涂层组假体周围的骨量面积比值平均分别为29．92％±3．92％、37．68％±3．61％、42．94％±4．15％，钛涂层组平均分别为29.36％±3．39％、36．35％±3．03％、41．71％±3.86％，同一组内术后不同时间点两两比较差异均有统计学意义（P〈0.05）；同一时间点两组比较差异均无统计学意义（P〉0．05）。结论随着植入时间的不断增加，钽涂层假体表面的成骨量不断增加；钽涂层假体具有良好的骨组织相容性及植入早期的稳定性。Objective To characterize bone biocompatibility and periprosthetic osteogenesis of Ta-coated prosthesis, Methods Sixty male New Zealand rabbits were randomized into a Ta-coated group （n = 30） and a titanium （Ti）-coated group （n = 30）. Ta-coated and Ti-coated prostheses were respectively implanted into the lempiral condyles of the animals in the 2 groups. Ten rabbits in each group were saerificed respectively at 4, 8, 16 weeks postoperation. Scanning electron microscopy （SEM） （2 samples）, energy-dispersive X-ray spectroscopy （EDX） （2 samples）, histopathological observation （8 samples）, histomorphometric analysis were employed to evaluate the volume of bone growth on the prosthetic surtace and surrounding the prostheses in both groups. Results At 4 weeks after implantation in both groups, SEM and EDX found attachment of bony tissue on the coating surface of prostheses and clear trabecular structure of the newly growing tissue. Histopathological observation demonstrated in both groups tight adherence of the new bony tissue to the prosthetic coating, stable coating without exfoliation and increased volume of periprosthetie osteogenesis with the increased implantation time. Bone morphometric analysis showed that the proportions of periprosthetic osteogenesis area at 4, 8 and 16 weeks after implantation were respectively 29.92%±3.92%、37.68%±3.61% and 42.94%±4.15% in the Ta-coated group and respectively 29.36%±3.39%、36.35%±3.03% and 41.71%±3.86% in the Ti-coated group. There were significant differences between any 2 time points within the same group （ P 〈 0. 05） but not between the 2 groups at the same time point （ P 〉 0.05）. Conclusions The periprosthetie osleogenesis of Ta-eoated prostheses increases with the time of implantation. Ta-eoated prostheses have good bone biocompatibility and demonstrate postsurgical stability at the early stage atier inlplantation.

关 键 词：钽 假体和植入 骨和骨组织 组织相容性 

分 类 号：R318.08[医药卫生—生物医学工程]