内毒素对大鼠牙髓组织形成修复性牙本质的影响  

Effect of lipopolysaccharide on the production of reparative dentin in rat dental pulp tissue

作　　者：于晓晨[1] 孙培[1] 邓婧[1] 潘克清[1] 

机构地区：[1]青岛大学医学院附属医院口腔内科,山东青岛266003

出　　处：《临床口腔医学杂志》2013年第4期209-210,共2页Journal of Clinical Stomatology

基　　金：青岛市科技支撑计划项目(1 0-3-3-3-6-nsh)

摘　　要：目的:探讨局部内毒素刺激对大鼠牙髓形成修复性牙本质的影响。方法:以内毒素注射于大鼠牙周组织,内毒素试剂盒检测血液中及逆行进入牙髓组织中的内毒素浓度,HE染色观察修复性牙本质形成情况。结果:血液中实验组与对照组间内毒素的含量差别有统计学意义,P<0.001。牙髓中实验组与阴性对照组,以及与空白对照组间内毒素的含量差别有统计学意义,P<0.001。HE染色可见注射位点附近修复性牙本质形成明显,髓腔变窄,牙本质小管排列紊乱。结论:内毒素在一定条件下能刺激大鼠牙髓组织,使其发生保护性反应,从而促进其矿化作用,加速修复性牙本质形成。Objective：Tu investigate the Effect of lipopolysaccharide on the production of reparative dentin in rat dental pulp tissue. Method： Rat periudontium was injected by Escherichia culi （Ee） lipopolysaccharide,and the concentra- lion of I,PS in blood and rat dental pulp tissue was detected by ehromogenie end-point tachypleus amebocyte lysate （CE TAI,） .The production of reparative dentin was observed by HE staining. Result：The concentration of LPS of the experimen- tal group was significantly higher than the concentration of control group in blood,P 〈0.0001：The coneentration of LPS of the experimental group was significantly higher than both negative control group and blank control group in dental pulp tis- sue, P 〈0.0001.HE staining showed that reparative dentin was produced nearby the injection site, where the dentinal tubule arrangment turned out to be rather disordered. Conclusion：The results indicate that LPS can stimulate dental pulp tissue to bring abut, t reactions of immune defense and promote the production of reparative dentin in certain situation.

关 键 词：内毒素 牙髓 修复性牙本质 

分 类 号：Q813.1[生物学—生物工程]