核转录因子-κB在糖尿病大鼠正中隆起室管膜细胞的表达  

NF-κB immuno-positive cells in the median eminence of diabetes mellitus rats

作　　者：张智琴[1] 郝庆卯[1] 马常升[1] 柳河[1] 曹翠丽[1] 

机构地区：[1]河北医科大学神经生物学研究室,石家庄050017

出　　处：《解剖学杂志》2013年第2期141-143,169,共4页Chinese Journal of Anatomy

基　　金：河北省自然科学基金（C2005000724,302491）;河北省卫生厅医学科学研究重点资助课题（20100032）

摘　　要：观察核转录因子-κB（NF-κB）在糖尿病大鼠不同时期正中隆起室管膜细胞的表达差异。方法：免疫组织化学观察NF-κB在2、4、8、11周的糖尿病大鼠及正常大鼠正中隆起处细胞内的表达情况。结果：NF-κB在2周组阳性表达水平最高，与正常对照组、4周、8周、11周比较差异有统计学意义；4周、8周组表达水平下降，但与11周、正常对照组比较差异有统计学意义；11周与正常组织之间无差异。结论：糖尿病时正中隆起处NF-κB阳性表达不同时期有差异，提示正中隆起作为敏感部位在糖尿病早期时，该处的NF-κB就被激活，参与了神经内分泌及神经免疫调节。Objective： To explore the expression difference of NF-κB in the cells of median eminence （ME） of diabetes meIli- tus rats at different time, and to explore the role in diabetes mellitus. Methods： Male Wistar rats were randomly divided into five groups： a control group; STZ-administered groups： 2-week group, 4-week group, 8-week group and ll-week group. The NF-κB immuno-positive cells of the above groups were observed with immunohistochemical method. Results： The value of optical density was high in the 2-week group, and the immuno-positive expression of the NF-κB was differ- ent from that in the control, the 4-week, 8 week and ll-week groups. The expression was decreased in the 4-week and 8-week groups, with a significant difference with that in the control and 11-week groups. Conclusion： During different stages of diabetes mellitus, the NF-κB expresses differently, suggesting the activation of the NF-κB in the early stages of diabetes mellitus in the ME. The ME may participate in the role of both neuroendocrine and the neural immune regulation.

关 键 词：核转录因子-ΚB 室管膜细胞 正中隆起 糖尿病 大鼠 

分 类 号：R692[医药卫生—泌尿科学]