经鼻及皮下给予睾丸酮／丙酸睾丸酮对大鼠相关脑区c-Fos表达效能的影响  

Effects of nasal administration or subcutaneous injection of testosterone/testosterone propionate on expression efficacy of c-Fos in rat brain

作　　者：张国梁[1] 牛小龙[1,2] 康云霄[1] 薛岩[1] 方卉[1] 石葛明[1] 

机构地区：[1]河北医科大学神经生物学研究室,石家庄050017 [2]河北大学医学部解剖学教研室,保定071000

出　　处：《解剖学杂志》2013年第2期192-196,共5页Chinese Journal of Anatomy

基　　金：河北省自然科学基金（C2012206033）

摘　　要：目的：探讨经鼻和皮下给予睾丸酮（T）或丙酸睾丸酮（TP）对大鼠中枢神经系统相关脑区激活的效能。方法：利用放射免疫分析法检测大鼠经鼻滴注给予TP后脑脊液和血清睾丸酮浓度的变化；以免疫组织化学观察大鼠各脑区c-Fos的表达。结果：放射免疫结果显示去势组大鼠脑脊液和血清中睾丸酮含量比正常组降低；去势大鼠皮下注射TP后只增加了血清中睾丸酮的含量，去势大鼠和正常大鼠经鼻给予TP后脑脊液和血清中睾丸酮的含量均明显增加；去势大鼠经鼻给予TP脑脊液中睾丸酮的含量高于去势皮下注射TP组，而血清中睾丸酮的含量低于去势皮下注射TP组。免疫组织化学结果显示正常大鼠鼻腔给予TP或睾丸酮增加大鼠多数脑区的c-Fos免疫反应阳性神经元数目和c-Fos免疫反应强度，而皮下注射TP只增加了少数脑区c-Fos免疫反应阳性神经元数目以及c-Fos免疫反应强度。结论：经鼻给予睾丸酮或TP后能够激活多数脑区c-Fos蛋白的表达，经鼻给予睾丸酮或TP的给药方式有可能成为治疗因雄激素低下导致的中枢神经系统疾病的一种有效手段。Objective： To explore the efficacy of intranasal administration or subcutaneous injection of testosterone （T） / tes tosterone propionate （TP） on different brain regions in rats. Methods： Radioimmunoassay was used to detect testosterone concentration in cerebrospinal fluid and in serum after intranasal administration of TP. Immunohistochemistry was used to de teet the expression of c-Fos protein in different brain regions. Results： Testosterone in cerebrospinal fluid and in serum was decreased in gonadectomized （GDX） rats compared to the normal control rats. Subcutaneous injection of TP in GDX rats only increased testosterone in serum. Testosterone in cerebrospinal fluid and in serum was increased after intranasal administration of TP in GDX rats and in the normal control rats. Testosterone in cerebrospinal fluid of GDX rats after intranasal administra- tion of TP was higher than that in GDX rats after subcutaneous injection of TP, however testosterone in serum of GDX rats after intranasal administration of TP was lower than that in GDX rats after subcutaneous injection of TP. After intranasal ad- ministration of TP or T, the number of c Fos-immunoreactive cells and the c-Fos immunoreactive intensity were increased in more brain regions. However, after subcutaneous injection of TP, the number of c-Fos-immunoreactive cells and the c-Fos immu noreactive intensity were increased only in a few brain regions. Conclusion： Intranasal administration of TP or T could induce the ex- pression of c-Fos and activate more brain regions. That can provide a new therapy method for some central nervous system diseases.

关 键 词：睾丸酮 丙酸睾丸酮 鼻腔给药 脑脊液 血清 C-FOS 

分 类 号：R972.1[医药卫生—药品]