神经肽Y、瘦素在Ⅱ型糖尿病大鼠垂体中的表达及丝胶的保护作用  

Expression of neuropeptide Y and leptin in hypophysis of type Ⅱ diabetic rats and the protective effects of sericin

作　　者：付秀美[1] 颜勇[1] 钟美蓉[1] 付文亮[1] 陈志宏[1] 薛景凤[1] 

机构地区：[1]承德医学院基础医学部解剖学教研室,承德067000

出　　处：《解剖学杂志》2013年第2期202-205,共4页Chinese Journal of Anatomy

基　　金：河北省教育厅项目（2006301）;河北省科技厅项目（08276101D-19）

摘　　要：目的：观察丝胶对Ⅱ型糖尿病大鼠垂体神经肽Y（NPY）和瘦素（1eptin）表达的影响。方法：雄性SD大鼠随机分为正常组、模型组、丝胶组和二甲双胍组。模型组、丝胶组和二甲双胍组均建立链脲佐菌素致Ⅱ型糖尿病模型，以血糖≥16．7mmol／L为成模标准；丝胶组和二甲双胍组分别于成模后给予丝胶和二甲双胍灌胃35d。采用酶法检测各组大鼠血糖、免疫组织化学显色和RT-PCR观察各组大鼠垂体NPY和瘦素及mRNA的表达。结果：与模型大鼠相比，丝胶组和二甲双胍组大鼠血糖、NPY和瘦素及mRNA表达明显降低，且两组比较无明显差别。结论：丝胶可下调垂体NPY和瘦素的高表达，对Ⅱ型糖尿病垂体损伤具有保护作用；且作用与二甲双胍相当。Objective： To investigate the effects of sericin on neusopeptide Y （NPY） and leptin expression in hypophysis of typeⅡ diabetic rats. Methods： Male SD rats were randomly divided into 4 groups： normal group, model group, sericin group and metformin group. The rats in the model group, seriein group and metformin group were given by continuous intraperitoneal injection of streptozotocin and blood glucose≥16.7 mmol/L was taken as the standard of modelformatioru After successfully establishing diabetes model, the rats in the sericin group and metformin group were lavaged with sericin and metformin for 35 d. The blood glucose was detected. Immunohistochemical staining and RT-PCR were used to detect the expression of NPY and leptin and their mRNA in hypophysis. Results： Compared with diabetes model rats, the blood glucose, the expressions of NPY and leptin and their mRNA in the sericin group and metformin group decreased significantly; Moreover, there were no remarkable differences between two groups. Conclusion： Sericin can down-regulate the high expression of NPY and leptin in hypophysis and has protective effects on hypophysis injury of type Ⅱ diabetes rats. Moreover, the effects of sericin on hy pophysis injury of type Ⅱ diabetes rats are similar with metformin.

关 键 词：丝胶 Ⅱ型糖尿病 垂体 神经肽Y 瘦素 

分 类 号：R587.1[医药卫生—内分泌]