松胞素B对人血淋巴细胞和CHL细胞微核率的影响  

THE EFFECTS OF CYTOCHALASIN B ON MICRONUCLEUS FREQUENCIES IN CHL CELLS AND HUMAN BLOOD LYMPHOCYTES

作　　者：孟紫强[1] 杜青平[1] 张波[1] 张玉清 王振瑞 张健彪 

机构地区：[1]山西大学环境医学与毒理学研究所,生命科学系,太原030006

出　　处：《应用与环境生物学报》2000年第4期341-343,共3页Chinese Journal of Applied and Environmental Biology

摘　　要：研究了不同浓度的松胞素B对人血淋巴细胞和中国仓鼠肺成纤维细胞 (CHL)分裂周期和微核率的影响 .结果表明 ,松胞素B浓度过高能引起细胞微核率增高 .在本研究中 ,对CHL细胞微核率试验以 3μg/mL松胞素B较适合 ;而对人血淋巴细胞微核试验 ,松胞素B浓度以 2～ 4μg/mL为宜 ,松胞素B浓度过高会提高微核背景值 ,降低试验的灵敏度和精确性 .表 4参On the basis of micronuclei (MN) formation theory, the effects of cytochalasin B at different concentrations on mitotic cycle and micronucleus frequencies of CHL cells and human blood lymphocytes were studied.The results showed that cytochalasin B at high concentrations could increase the micronucleus frequencies in two kinds of cells, and could also increase the background of MN frequency in control group and decrease the sensitivity and accuracy of the experiments. So the favorite concentration of cytochalasin B is 3 μ g/mL for CHL cell micronuclei experiments and 2～4 μ g/mL for human blood lymphocyte. Tab 4, Ref 9

关 键 词：人血淋巴细胞 CHL细胞 松胞素B 微核 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]