养殖牙鲆淋巴囊肿病病原的研究  

Study on the Causative Agent of Lymphocystic Disease in Cultured Flounder, Paralichthys Olivaceus, in China's Mainland

作　　者：徐洪涛[1] 朴春爱[2] 姜忠良 王文兴[3] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]TAMU [3]国家海洋局第一海洋研究所,山东青岛266003

出　　处：《病毒学报》2000年第3期223-226,共4页Chinese Journal of Virology

摘　　要：近几年我国海水养殖鱼类发生病毒性传染病 ,病鱼鳍、鳃及体表皮肤出现乳头瘤样赘生物。将发病牙鲆(Paralichthysolivaceus) 表皮瘤组织进行超薄切片 ,电镜下在病变组织明显肥大的细胞胞浆内发现大量球状病毒 ,呈二十面体对称 ,具包膜 ,直径约 2 10nm。根据虹彩病毒主要衣壳蛋白 (majorcapsidprotein ,MCP)基因中间保守区序列设计简并引物 ,应用PCR方法从病变组织中扩增出长 6 36bp的片段。经DNA序列测定及同源性分析表明 ,该段核苷酸序列与欧洲分离的淋巴囊肿病病毒 1型 (lymphocysticdiseasevirustype 1,LCDV - 1)MCP基因相应序列有 77%的同源性 ,表明该病毒属虹彩病毒科 (Iridoviridae)淋巴囊肿病毒属 (Lymphocystivirus) ,但与欧洲分离株有一定差异。将该病毒暂命名为淋巴囊肿病病毒中国分离株 (lymphocystisdiseasevirus-Chineseisolate ,LCDV -cn)。Viral epizootic causing papilloma like lesions on the skin, gill and fin of factory cultivated flatfish, Paralichthys olivaceus, occurred in China's Mainland and caused great economic loss in the past few years. Electron microscopy showed large amount of enveloped icosehedral, hexogonal viruses in the cytoplasm of hypertrophied cells. The diameter of the virus averages in 210 nm (side to side) . A pair of degenerate primers based on the conserved sequence of the major capsid protein (MCP) gene of iridovirus amplified a 636bp fragment using DNA extracted from the virus purified from diseased fish tissues as the template. The nucleotide sequence of the amplified fragment showed 77% homology with the corresponding region of the MCP gene of lymphocystic disease virus type 1 (LCDV-1) . Both elcectron microscopy and DNA sequencing results showed that this virus belongs to genus Lymphocystivirus, family Iridoviridae. The virus reported here was named temporally the Chinese flounder isolate of lymphocystic disease virus (LCDV-cn) . This is the first report on the partial DNA sequencing of the MCP gene of piscine iridovirus isolated in China's Mainland.

关 键 词：牙鲆 虹彩病毒 淋巴囊肿病 DNA序列测定 

分 类 号：S852.659.1[农业科学—基础兽医学]