原位杂交研究对虾白斑杆状病毒在虾体内感染过程  

Study of White Spot Syndrome Baculovirus Infection Process in Penaeus Monodon by in situ Hybridization

作　　者：黄灿华[1,2] 石正丽[2,5] 张建红[2] 张吕平[3] 吴雄飞 J.R.Bonami 陈棣华[2] 吴清江[1] 

机构地区：[1]中国科学院水生生物研究所 [2]中国科学院武汉病毒研究所无脊椎动物病毒学联合开放实验室,湖北武汉430071 [3]中国科学院南海海洋研究所 [4]宁波市水产研究所 [5]UMR219,CNRS-IFREMER DRIM Place E.Bataillon,cp-80 34095 Montpellier,Cedex 5,France

出　　处：《病毒学报》2000年第3期242-246,共5页Chinese Journal of Virology

基　　金：中国科学院重大B项目!(K2 95 1-13 1-111)

摘　　要：应用地高辛标记的对虾白斑杆状病毒 (whitespotsyndromebaculovirus,WSSV)核酸探针 ,与人工感染后不同时间采集的对虾组织样品进行原位杂交 ,以动态研究病毒从侵染至对虾发病死亡的过程。将典型感染WSSV的病虾组织投喂健康对虾 ,结果显示 :WSSV首先通过侵染消化道上皮进入虾体内增殖 ,此后随着细胞裂解、病毒粒子释放 ,游离的病毒粒子伴随血淋巴循环进而侵染其它靶组织 ,直至对虾发病死亡。The tissue sections of artificially infected shrimp (Penaeus monodon) sampled at different times were hybridized in situ with digoxigenin (DIG)-labeled WSSV DNA probe in order to dynamically study the viral infection process in shrimp body. Feeding the healthy Penaeus monodon with diseased shrimp tissues which was confirmed to be typically infected with WSSV. The results showed that WSSV firstly infected and replicated in the epithelial cells of alimentary tract, the viral particles then released with the host cell lysis. The dissociative viral particles proceeded to infect other target tissues following hemolymph circulating in the shrimp body till the shrimp got diseased or died.

关 键 词：原位杂交 对虾白斑杆状病毒 斑节对虾 杂过程 

分 类 号：S945.47[农业科学—水产养殖] S941.41[农业科学—水产科学]