盐酸吡格列酮对高糖培养的肾小球MCs氧化应激的影响  

Effects of pioglitazone on high glucose mediated oxidative stress in cultued rat glomerular mesangial cells

作　　者：孙文佳[1] 叶山东[1] 刘皆[1] 胡圆圆[1] 

机构地区：[1]安徽省立医院内分泌科,安徽合肥230001

出　　处：《中华疾病控制杂志》2013年第4期295-298,共4页Chinese Journal of Disease Control & Prevention

基　　金：安徽省自然科学基金(11040606M161);安徽高校省级自然科学研究项目(KJ2011A157)

摘　　要：目的观察盐酸吡格列酮对高糖培养的肾小球系膜细胞(mesangial cells,MCs)氧化应激的影响,探讨其肾脏保护机制。方法体外培养MCs随机分组为正常糖(normal glucose group,NG)组、高糖(high glucosegroup,HG)组及高糖+不同浓度吡格列酮(P1、P2、P3)组。培养48 h后,收集各组细胞及上清液。以2',7'-二氯双氢荧光素二乙酸酯(2',7'-dichlorofluorescin diacetate,DCFH-DA)为荧光探针,采用流式细胞仪法检测MCs内活性氧(reactive oxygen species,ROS)水平。采用比色法检测MCs上清液中的脂质过氧化物丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性。结果与NG组比较,高糖刺激后MCs内ROS水平明显增加,上清液中MDA含量显著增高、SOD活性下降,差异有统计学意义(P<0.001);吡格列酮干预后可明显抑制高糖刺激下的MCs上述变化,且呈浓度依赖性。结论盐酸吡格列酮可抑制高糖诱导的大鼠MCs氧化应激,且呈浓度依赖性,该作用可能部分与其肾脏保护有关。Objective To observe the effects of pioglitazone on high glucose mediated oxidative stress in cultured rat glomeular mesangial cells and investigate its reno-protective effect. Methods Rat glomerular mesangial cells were cul- tured in the medium with normal glucose（NG） group, high glucose（HG） group and different concentrations of pioglitazone （group P1,1＇2 ,P3 ）. After 48h exposure, the MCs and supernatants were collected. The standards of Intracellular reactive oxygen species （ROS） were detected by flow cytometry, which used 2＇ ,7＇ -dichlorofluorescein diaeetate （DCFH-DA） as a reactive oxygen species fluorescent probe. Coloimetry method was used to detect the levels of malondialdehyde（MDA） and superoxide dismutase （ SOD）o Results （ 1 ） Compared with group NG, the levels of intracelluar ROS and malondialdehyde in the supernatants were significantly increased in group HG, while SOD activity was decreased markedly（P 〈 0. 001 ）; （2） Pioglitazone inhibited siginificantly intraeelluar ROS generation and malondialdehyde levels in the supernatants and in- creased SOD activity in the supernatants in a dose-dependent manner. Conclusions High glucose-induced oxidative stress can be suppressed by pioglitazone in a dose-dependent manner in glomerular mesangial cells, which may partly relevant to its reno-protection.

关 键 词：肾小球 对比研究 糖尿病肾病 

分 类 号：R181.3[医药卫生—流行病学]