盐胁迫诱导的植物细胞凋亡——植物抗盐的可能生理机制  

SALT STRESS-INDUCED APOPTOSIS IN PLANTS——A POSSIBLE RESISTANCE MECHANISM TO SALT STRESS

作　　者：宁顺斌[1] 宋运淳[1] 王玲[1] 李霞[1] 

机构地区：[1]武汉大学发育生物学研究中心,武汉430072

出　　处：《实验生物学报》2000年第3期245-253,共9页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(批准号;39870423);国家教委博士点基金~~

摘　　要：近来的研究表明,一定条件的盐胁迫可导致植物细胞程序性死亡。本文利用DNA Lad-dering、石蜡切片原位检测以及染色体涂片原位检测,从组织、细胞以及DNA等多个方面对盐胁迫下的玉米、水稻和烟草根尖细胞死亡作了研究,形态特别是生化方面的证据表明盐胁迫诱导的植物细胞凋亡可能在植物界具有一定的普遍性。但各个物种之间有一定差异。本实验结果对盐胁迫下的植物生理机制提供了新的研究思路。同时,我们还对基于染色体制片和石蜡切片的原位检测方法进行了比较和讨论。我们认为,基于染色体制片的原位标记技术适合于定性和定量检测单个细胞的凋亡,具有一些石蜡切片所不可及的优点。Previous study has showed that apoptosis-like cell death can be induced under moderate salt stress in plants. In this paper, we studied the cell death induced by salt stress in maize, rice and tobacco roots utilizing DNA Laddering, paraffin sectioning-based TUNEL and chromosome spreading-based TUNEL simultaneously. The characteristic morphological and biochemical features showed apoptosis induced by salt stress may be a universal phenomenon in plants, but some differences may lie in various species. These results provided a valuable insight into studying the physiological mechanism of stress resistance in plants. In addition, we compared the in situ labelling technique based on chromosome spreading with that based on paraffin sectioning. We proposed according to the results that chromosome spreading-based in situ labeling technique should be suitable to detect individual cell death qualitatively and quantitatively with high efficiency, low background and detailed description of apoptotic changes at chromosome, nuclear and DNA levels.

关 键 词：盐胁迫 植物细胞调亡 植物抗盐 生理机制 

分 类 号：Q945[生物学—植物学]