类整合素在烟草花粉萌发和花粉管生长中的作用  

THE EFFECT OF INTEGRIN-LIKE PROTEINS ON POLLEN GERMIANTION AND TUBE GROWTH OF NICOTIAN A TABACUM

作　　者：孙颖[1] 韩晔[1] 赵军峰 白娟[1] 郝美璞 孙大业[1] 

机构地区：[1]河北师范大学生物系,石家庄050016

出　　处：《实验生物学报》2000年第3期255-262,共8页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(批准号:39970071);河北省教委博士基金~~

摘　　要：本文研究了动物整合素VnR抗血清及动物整合素专一性抑制剂含RGD的多肽对体外及半体内培养条件下烟草花粉萌发及花粉管生长的影响。结果表明:在体外培养条件下,VnR抗血清及GRGDSP肽对花粉的萌发及花粉管的生长没有明显的抑制作用,但可抑制钙调素促进的花粉萌发和花粉管的生长;两者对柱头上进行的花粉萌发及在花柱里进行的花粉管生长也有一定程度的抑制。对类整合素在花粉萌发及花粉管生长中的作用进行了讨论。The effects of antiserum against human VnR integrin and integrin antagonist GRGDSP pep-tides on Nicotiana tabacum pollen germination and tube growth both in in vitro and in semi-vivo conditions were studied. No obvious inhibitiory effects on pollen germination and tube growth in vitro were observed when anti-VnR serum or GRGDSP peptides was added to BK culture medium, but the enhancement of pollen germination and tube growth in vitro promoted by calmodulin was depressed by adding anti-VnR serum or GRGDSP peptides to BK culture medium. In addition to that, pollen germination and tube growth on stigma, as well as tube growth in styles were also inhibited at some extent by treating stigma and microinjecting GRGDSP peptides or anti-VnR serum into styles. 1 he role of integrin-like proteins in regulation of pollen germination and tube growth in situ was discussed.

关 键 词：类整合素 烟草花粉萌发 花粉管生长 

分 类 号：S572[农业科学—烟草工业]