评价植物悬浮细胞培养物两项测定指标的建立(简报)  

THE ESTABLISHMENT OF TWO NEW EVALUATION CRITERIA FOR CELL STATE OF PLANT CELL SUSPENSION CULTURES

作　　者：陈葆棠[1] 谢岳峰[1] 张端品[1] 余毓君[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《实验生物学报》2000年第3期283-284,共2页Acta Biologiae Experimentalis Sinica

基　　金：国家"九五"生物技术攻关计划资助

摘　　要：由于在细胞培养研究中缺乏一些可操作性强的且定量化的细胞状态评价指标,人们对植物细胞状态的有些性状的评价只能停留在定性描述水平,如对悬浮细胞培养物褐化程度的评价仅能作出定性判断。这里我们提出了两项悬浮细胞培养物细胞状态评价指标。An experiment on suspending rice cell cultures in sucrose solution and on optical density (OD) value in culture solution at 576 nm wave width was carried out. the result indicates that suspension rate ( % ) of suspensions. while suspending in 0. 76 mol/L sucrose solution for 15 minutes at room temperature, can be employed as an judgment criterion of cell state. Suspending cell suspension in 0. 76 mol/L sucrose solution is also a good approach on selecting desirable cells from suspension cell mass, as a result, it is beneficial to establishing cell suspension. The browning of cell suspension is related to the necrosis degree of suspension cells. OD value of cell suspension liquid at 576nm can be taken as a criterion with which the necrosis degree of cell suspension can be evaluated.

关 键 词：植物悬浮细胞培养物 上飘率 光密度值 

分 类 号：Q943.1[生物学—植物学]