斜纹夜蛾核多角体病毒 (SpltNPV)基因组4 .73kb片段的序列分析  

Nucleotide Sequence of a 4 730 Base Pairs Region of the Spodoptera litura Nucleopolyhedrovirus Genome

作　　者：郑姬[1] 李镇[1] 龙綮新[1] 魏永杰[1] 王珣章[1] 

机构地区：[1]中山大学昆虫学研究所,生物防治国家重点实验室,广州510275

出　　处：《生物化学与生物物理学报》2000年第5期445-450,共6页

基　　金：国家自然科学基金重点项目资助!No .3 973 0 0 3 0&&

摘　　要：报道了斜纹夜蛾核多角体病毒 (Spodopteralituranucleopolyhedrovirus ,SpltNPV)基因组EcoRI E片段中长 4730bp的序列分析结果。该序列上含有完整的SpltNPVodv e6 6基因 ,一个开放读码框ORF 10 86 (AcMNPVORF10 9的同源区 )和一个类似亮氨酸拉链蛋白的基因 (命名为 p34基因 )。序列比较分析表明 ,SpltNPVodv e6 6基因编码区全长 2 0 79bp ,编码一种 6 92aa的蛋白质 ,其起始密码子ATG上游只有一个常见的晚期基因启动子模体 (motif)ATAAG而基因的下游无典型的poly(A)加尾信号。SpltNPVODV E6 6蛋白质的氨基酸序列与LdMN PV、LsNPV、AcMNPV、BmNPV、CfNPV、OpMNPVODV E6 6蛋白质具有四个高度保守的区域 ,其上含有一推断的核定位信号KKIWAEDGR。odv e6 6基因上游的ORF与其同向不同框 ,5′端除有CAAT盒 ,还发现有典型的杆状病毒早期基因启动子元件ACGT和GC单元以及晚期基因的转录起始核心序列TTAAG。预测的ORF产物为2 89个氨基酸 ,分子量为 33771的蛋白质 ,故命名为P34蛋白。SpltNPVP34蛋白的C端和N端分别有一个亮氨酸拉链结构和类亮氨酸拉链结构。SpltNPV p34基因的调控元件可能具有重要功能。odv e6 6基因 3′端 5 14nt处的ORF 10 86方向与odv e6 6基因相反 ,能编码一种 36 1aa的多肽。ORF 10A 4 730 bp region of the Spodoptera litura nucleopolyhedrovirus genome Eco RI-E fragment was sequenced, in which the odv-e66 gene and other two ORF s, ORF 1086 and the p34 gene, were found. The SpltNPV odv-e66 gene op en reading frame was deduced to be 2 079 bp, encoding a protein of 692 amino aci ds. Unlike odv-e66 genes of AcMNPV and LsNPV, only one transcriptio nal initiation conserved sequence ATG of the late gene was present upstream of the initial codon ATG of SpltNPV odv-e66 gene, and no poly(A) signal w as detected at the 3′ end of the gene. Four highly conserved regions homologous to those of LdMNPV, LsNPV, AcMNPV, BmNPV, CfNPV and OpM NPV were present in the amino acid sequence of SpltNPV ODV-E66 protein, with t he putative nuclear targeting signal(NTS) KKIWAEDGR in the fourth conserved regi on. The ORF upstream of the odv-e66 gene had the same direction of odv- e66 gene, but different reading frame. Besides the CT box, the early gene regulatory element ACGT and GC motifs and the transcriptional initiation conserv ed sequence TTG of the late gene were present at the 5′ end of this ORF. A pu tative protein of 289 amino acid residues with Mr. about 34 kD was encoded b y the ORF, so that it was named P34 protein. A leucine zipper and a leucine zipper-l ike structure were found in the C- and N-termini of SpltNPV P34 protein, re spectively. The functions of those regulatory elements may be of great importanc e to SpltNPV genome. An 1 086 nt open reading frame (ORF) capable of enc odin g a 361 amino acids polypeptide was found at the 3′ end of the odv-e66 gen e and in opposite direction. The ORF 1 086 protein shares 44% amino acid sequenc e homology with AcMNPV ORF 109.

关 键 词：斜纹夜蛾核多角体病毒 odv-c66基因 ORF1086 p34 

分 类 号：Q754[生物学—分子生物学] Q939.403