斜纹夜蛾核多角体病毒几丁质酶基因的克隆及序列分析  

Cloning and Sequence Analysis of the Chitinase Gene of Spodoptera litura Nuclear Polyhedrosis Virus

作　　者：胡国栋[1] 庞义[1] 杨凯[1] 李充璧[1] 苏德明[2] 

机构地区：[1]中山大学生物防治国家重点实验室/昆虫学研究所,广州510275 [2]复旦大学生命科学学院病毒学研究室,上海200433

出　　处：《生物化学与生物物理学报》2000年第5期537-540,共4页

基　　金：国家自然科学基金!(No .3 973 0 0 3 0和No .3 9680 0 2 9)资助项目&&

摘　　要：以苜蓿丫纹夜蛾核多角体病毒 (Autographacalifornicamultinucleocapsidnuclearpolyhedrosisvirus,AcMN PV)基因组含几丁质酶基因 (chiA)的 pstIM片段为探针 ,通过Southern杂交将斜纹夜蛾核多角体病毒(Spodopteralituranuclearpolyhedrosisvirus,SpltNPV)的chiA基因定位在其基因组DNA两个相邻的XbaI片段(5 .1kb及 2 .1kb片段 )上 ,克隆了包含该基因完整序列的片段 ,并测定了它的核苷酸全序列。该基因读码框为16 95个核苷酸 ,编码 5 6 4个氨基酸的蛋白质 ,分子量为 6 2 .9kD ,在起始密码子ATG上游 - 10处有一个杆状病毒晚期启动子模体TAAG ,在终止密码子TAA下游有一个真核生物基因mRNA转录poly(A)加尾信号序列AATAAA。在SpltNPVchiA基因氨基酸序列的N端预期有由 2 3个氨基酸构成的真核生物信号肽。SpltNPVchiA基因氨基酸序列与AcMNPVchiA基因序列的同源性为 5 7%。Using AcMNPV chiA-containing fragment as a probe, the chitinase gene of Spodoptera litura nuclear polyhedrosis virus (SpltNPV) was localized in two contiguous fragments, XbaI 5.1 kb and 2.1 kb, and the intact SpltNPV chi A gene w as obtained by cloning and sequencing those two fragments. The complete open rea ding frame of the gene was 1 695 nucleotide long, encoding a putative protein of 564 amino acids with molecular weight of 62.9 kD. Sequence analysis further rev ealed that the 5′ noncoding region had a baculovirus late promoter motif TG. A polyadenylation signal, TA, was located dowmnstream of the translation sto p codon. A putative signal peptide was present at the N-terminus of the protein . This gene shares 57% homology with AcMNPV chitinase gene.

关 键 词：斜纹夜蛾 核多角体病毒 几丁质酶基因 序列分析 

分 类 号：Q785[生物学—分子生物学] S433.3[农业科学—农业昆虫与害虫防治]