用RDA技术寻找肝再生相关基因的初步研究  

Isolation of regulation genes related with liver regeneration by representational difference analysis

作　　者：许望翔[1] 汪思应[1] 魏汉东[1] 杨晓明[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《生理学报》2000年第4期277-277,共1页Acta Physiologica Sinica

基　　金：TheprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No 396 70 36 6 )

摘　　要：利用表达性差异显示分析 (RDA)技术 ,研究大鼠 2 /3肝部分切除后 1h肝组织中基因的选择性表达 ,建立了大鼠 2 /3肝部分切除术后 1h再生肝组织选择性表达基因EST库。该库约含 3× 10 4 个独立克隆 ,其中 95 %以上的克隆含有插入片段 ,长度约 2 0 0～ 70 0bp不等 ,对随机挑出的 5 2个克隆的序列分析表明其中大多数基因与肝再生调控相关 (38/5 2 )。 10株未报道序列经RNA杂交证实 ,其中 6株与肝再生相关。mRNA isolated from 2/3 partially hepatectomied rats was used as a “tester” for representational difference analysis (RDA). The subtracted tester cDNA was cloned into a T vector and a rat regeneration liver specific EST pool was constructed, which contained about 3×10 4 independent clones. A sequence analysis of 52 clones randomly picked up from this pool indicated that the liver regeneration specific sequences were enriched, and the results of RNA blots revealed some novel genes in association with liver regeneration.

关 键 词：肝再生 表达性差异显示分析 RDA 基因克隆 

分 类 号：R334[医药卫生—人体生理学]