周围神经损伤后外源性GDNF对神经元的保护作用  

Protective effect of exogenous glial cell line-derived neurotrophic factor on neurons after sciatic nerve injury in rats

作　　者：陈哲宇[1] 曹莉[1] 路长林[1] 何成[1] 鲍璿[2] 

机构地区：[1]第二军医大学神经生物学教研室,上海200433 [2]中国科学院上海脑研究所,上海200031

出　　处：《生理学报》2000年第4期295-300,共6页Acta Physiologica Sinica

基　　金：ThisworkwassupportedbytheChineseNationalFundamentalBasicResearchProject (No 19990 5 40 0 5 )

摘　　要：采用硅管套接大鼠切断的坐骨神经模型 ,局部给予胶质细胞源性神经营养因子 (GDNF) ,应用尼氏染色、酶组织化学染色方法 ,观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目 ,降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶 (CHE)及酸性磷酸酶 (ACP)变化的幅度。这表明外源性GDNF能保护周围神经切断后引起的神经元损伤。The aim of the study was to investigate the effect of exogenous glial cell line derived neurotrophic factor (GDNF) on spinal cord neurons after sciatic nerve axotomy. Upon silicone tubulization of transected sciatic nerve in the adult rat, either 0 9% saline or GDNF solution was injected into the silicone chamber. It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions. These results suggest that exogenous GDNF is capable of protecting motor neurons from death induced by peripheral nerve injury.

关 键 词：周围神经损伤 神经元 GDNF 保护作用 

分 类 号：R338.1[医药卫生—人体生理学]