高粱雄性不育系热激前后线粒体的变化与育性的关系  

The Relationship Between the Change of Mitochondria and the Fertility in CMS Line of Sorghum After Heat Shock

作　　者：王俐[1] 祁新红[1] 刘根齐[1] 章银梅[1] 陈建南[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《Acta Genetica Sinica》2000年第9期834-838,共5页

基　　金：国家自然科学基金!(批准号:39780026)

摘　　要：高粱雄性不育系3197A（3A）在花粉母细胞期进行热激处理后由不育变为可育。提取其处理小穗线粒体用流式细胞仪计数进行分析。结果发现经热激处理后，3A不育系的线粒体数目由1.55×106个／mg鲜穗增加至7.1×106／mg。同时对热激处理后3A的花粉育性及同工酶进行了研究，发现热激后3A的花粉粒变的饱满并可被I2朘I 溶液染色，其细胞色素氧化酶和过氧化物酶的电泳酶谱中出现与保持系3B一致的酶带。The mitochondria of anther in pollen mother cell stage of cytoplasmic male sterility (CMS) line 3197A in sorghum were extracted after heat shock. The number of the mitochondria was measured by flow cytometer and some isoenzymes were analyzed by PAGE combined with stain for enzyme activity. The result of mitochondria quantity measurement shows that the number of the mitochondria in CMS line 3197A increased greatly up to 4.5 folds after heat shock. The analysis of isoenzymes also demonstrates that the type of isoenzyme in CMS line is in consistent with maintainer line. The study denoted the change of the quantity of mitochondria after heat shock and the relationship between this change and the fertility in sorghum.

关 键 词：高梁 细胞质雄性不育 热激 线粒体 育性恢复 

分 类 号：Q943[生物学—植物学]