低温下强光胁迫对小麦叶片光系统Ⅰ结构与功能的影响  

Effects of Strong light Stress at Low Temperature on Structure and Function of Photosystem Ⅰ from Wheat ( Triticum aestivum L.) Leaves

作　　者：陈国祥[1] 何兵[1] 魏锦城[1] 张荣铣[2] 

机构地区：[1]南京师范大学生物系,南京210097 [2]南京农业大学农学系,南京210095

出　　处：《植物生理学报（0257-4829)》2000年第4期337-342,共6页Acta Phytophysiologica Sinica

基　　金：国家重点基础研究专项经费!(No .G1998010100);江苏省自然科学基金资助项目

摘　　要：探讨了低温下强光胁迫对小麦 (TriticumaestivumL .)扬麦 5号苗期叶片 (第三叶 )类囊体膜上PSⅠ 2 0 0颗粒光化学特性和组分的影响。光胁迫条件下 ,PSⅠ 2 0 0颗粒的电子传递活性受到抑制 ,4℃条件下光胁迫的抑制程度低于 2 5℃下的抑制。 2 5℃条件下 ,光胁迫导致PSⅠ 2 0 0颗粒还原侧 19kD及LHCⅠ等多肽降解 ;而 4℃条件下 ,19kD保持稳定 ,但LHCⅠ等多肽在光胁迫处理过程中却发生降解。根椐实验结果推测 ,在低温光胁迫条件下 。The effects of strong light stresses at low temperature on the functional and structural characteristics of photosynthetic membranes (thylakoid membranes, membranes in the PSⅠ and PSⅡ particles) from wheat ( Triticum aestivum L.) Yangmai No.5 seedling leaf (third leaf) were studied by measuring the changes in electron transport activities, fluorescence emission spectra and analyzing the composition of membrane protein polypeptide components. Under low temperature and strong light stresses, the electron transport activities of the thylakoid membrane and PSⅠ 200 particles in seedling leaves were inhib[HJ6”:2]ited. The extent of inhibition by light stress treatment was more serious at 25℃ than at 4℃ (Fig.1). Light stress at 25℃ induced degradation of the 19 kD polypeptide on the acceptor side of PSⅠ 200 particles and LHCⅠ protein. At 4℃, polypeptide of LHCⅠ was also degraded by light stress treatment, but the 19 kD component remained stable(Fig.2). It is suggested that LHCⅠ is essential for the stability of reaction center structure of the PSⅠ complex.

关 键 词：小麦 低温 强光胁迫 光系统I 

分 类 号：Q945.11[生物学—植物学]