碲化镉量子点对大鼠骨髓间充质干细胞增殖与成骨分化潜能的影响  

Effect of CdTe QDs on proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

作　　者：周煜博[1] 郭丽[1] 孟春阳[1] 李鹏[1] 卢日峰[1] 杨小玉[2] 尹飞[3] 

机构地区：[1]吉林大学公共卫生学院毒理学教研室,吉林长春130021 [2]吉林大学中日联谊医院骨科,吉林长春130033 [3]吉林大学第一医院脊柱外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第2期213-217,431,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30972153/C180103);吉林省卫生厅医学科研基金资助课题(2009Z044)

摘　　要：目的:探讨碲化镉量子点(CdTe QDs)对大鼠骨髓间充质干细胞(BMSCs)增殖与成骨细胞分化潜能的影响,阐明CdTe QDs的体外毒性,并为CdTe QDs作为BMSCs体外活细胞标记应用提供实验依据。方法:荧光光谱法检测CdTe QDs在DMEM/F-12培养液中的分散情况。大鼠原代培养BMSCs,经不同浓度CdTe QDs(0、0.195 3、0.390 6、0.781 3、1.562 5、3.125 0、6.250 0、12.500 0、25.000 0和50.000 0nmol.L-1)作用24h后,MTT法检测CdTe QDs对BMSCs增殖的影响。体外地塞米松、甘油磷酸钠和维生素C诱导BMSCs向成骨细胞分化,茜素红染色显示钙结节。计算半数增殖抑制浓度(IP50)和成骨半数分化抑制浓度(ID50)。结果:荧光光谱法检测,CdTe QDs在DMEM/F-12培养液中分散良好,未发生聚合。随着BMSCs暴露于CdTeQDs的时间延长,其细胞的生长逐渐减慢,暴露24、48和72h的回归系数分别为-23.96,-29.61和-24.30(P<0.05),IP50分别为7.25、1.63和0.67nmol.L-1。随着CdTe QDs浓度的增加,BMSCs分化成的成骨细胞逐渐减少,暴露48h的成骨分化回归系数为-56.15(P<0.05),ID50为0.0412nmol.L-1,增殖分化抑制比(IP50/ID50)=39.56。结论:在一定浓度范围内(0.195 3、0.390 6、0.781 3、1.562 5、3.125 0、6.250 0、12.500 0、25.000 0和50.000 0nmol.L-1),CdTe QDs抑制BMSCs的增殖;在一定浓度范围内(0.012 2、0.024 4、0.048 8、0.097 6和0.195 3nmol.L-1,CdTe QDs抑制BMSCs向成骨细胞的分化。在使用CdTeQDs作为活细胞标记物时,需要考虑其对细胞增殖及分化的影响。Objective To explore the effect of cadmium telluride quantum dots（CdTe QDs） on the proliferation and osteogenesis differentiation of rat bone marrow mesenchymal stem cells（BMSCs）,and to illustrate the toxicity of CdTe QDs in vitro,and to provide experimental evidence for application of CdTe QDs for living cell labeling marker.Methods Photoluminescence detector was used to detect photoluminescence emission spectra of the CdTe QDs in order to determine the dispersion of CdTe QDs in DMEM/F-12 medium.The rat BMSCs were cultured,and MTT assay was performed to examine the effects of different concerntrations（0,0.195 3,0.390 6,0.781 3,1.562 5,3.125 0,6.250 0,12.500 0,25.000 0,and 50.000 0 nmol·L-1） of CdTe QDs on the proliferation of BMSCs;in vitro hexadecadrol,sodium glycerophosphate and vitamin C were used to induce osteogenesis differentiation.Alizarin red staining was used to display calcium nodus.The concentration inducing 50% of inhibition of proliferation（IP50） and the concentration inducing 50% of inhibition of differentiation（ID50） were calculated.Results Photoluminescence emission spectra showed that CdTe QDs were well dispersed in DMED-F/12 medium without segregation.The longer the exposure time went,the less the cell viability remained.The regression coefficients of 24,48 and 72 h exposure were separately-23.96,-29.61 and-24.30（P0.05）;and the IP50 of each time was separately 7.25,1.63,and 0.67 nmol·L-1.And the higher the exposure dose was,the lower the osteogenesis differentiation rate was.The regression coefficient of 48 h exposure was-56.15（P0.05）,and the ID50 was 0.041 2 nmol·L-1,and the ratio of IP50/ ID50 was 39.56.Conclusion At a certain concentration range（0.012 2-50.000 0 nmol·L-1）,CdTe QDs could inhibit the proliferation and osteogenesis differentiation of BMSCs.When CdTe QDs are used as living cell labeling marker,their influence in cell proliferation and differentiation should be considered.

关 键 词：碲化镉量子点 骨髓间充质干细胞 细胞增殖 细胞分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]