吲哚美辛联合X线照射对人急性髓系白血病HL-60细胞的增殖抑制作用  

Inhibitory effect of indomethacin combined with radiation on proliferation of human acute myeloid leukemia HL-60 cells

作　　者：王波[1,2] 顾俊莲[2] 戴玉鑫 佟力军 孟令娜 李扬[2] 

机构地区：[1]内蒙古林业总医院病理科,内蒙古呼伦贝尔022150 [2]吉林大学白求恩医学院病理生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第2期218-221,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(81260310)

摘　　要：目的:观察吲哚美辛联合X线照射对人急性髓系白血病HL-60细胞的增殖抑制作用,为抗肿瘤研究提供依据。方法:将HL-60细胞培养于含吲哚美辛的培养基中,终浓度分别为0、20、40、60、80和100μmol.L-1。同时给予3Gy X线照射,继续培养24h;MTT法检测细胞增殖抑制率;台盼蓝染色法检测细胞活力;实时荧光定量PCR检测细胞增殖和凋亡相关基因PCNA和Caspase-3mRNA表达变化。结果:与对照组比较,不同浓度吲哚美辛联合照射组HL-60细胞增殖抑制率明显增加,80μmol.L-1吲哚美辛联合照射组HL-60细胞增殖抑制率最高(P<0.01)。与对照组、吲哚美辛(80μmol.L-1)组和照射组比较,80μmol.L-1吲哚美辛联合照射组HL-60细胞活力抑制程度最高(P<0.05或P<0.01)。与对照组、吲哚美辛(80μmol.L-1)组和照射组比较,80μmol.L-1吲哚美辛联合照射组PCNA mRNA表达水平明显降低(P<0.01),Caspase-3mRNA表达水平明显升高(P<0.01)。结论:吲哚美辛可显著增强照射对HL-60细胞的增殖抑制作用。Objective To investigate the inhibitory effect of indomethacin combined with radiation on proliferation of HL-60 cells and to provide basis for study on antitumor therapy.Methods The HL-60 cells were exposed to indometnacin at 0,20,40,60,80,and 100 μmol·L-1 together with 3 Gy X-rays radiation and cultivated for 24 h.MTT and Trypan blue exclusion experiments were used to detect the inhibitory rate of cell proliferation and viability respectively.Real-time PCR was used to detect the changes of cell proliferation and apoptosis-related gene PCNA and Caspase-3 mRNA expressions.Results Compared with control group,the inhibitory rates of proliferation of HL-60 cells in different doses of indomethacin groups were increased significantly（P0.01）,especially in 80 μmol·L-1 indomethacin group.Compared with control,indomethacin（80 μmol·L-1） and 3 Gy X-rays radiation groups,the inhibitory rate of cell viability of HL-60 cells in 80 μmol·L-1 indomethacin combined with radiation group was increased significantly（P0.01）.Compared with control,indomethacin（80 μmol·L-1） and 3 Gy X-rays radiation groups,the expression of PCNA mRNA in 80 μmol·L-1 indomethacin combined with radiation group was decreased and the expression of Caspase-3 mRNA was increased significantly（P0.01）.Conclusion Indomethacin can enhance the inhibitory effect of radiation on proliferation of HL-60 cells.

关 键 词：吲哚美辛 照射 HL-60细胞 细胞增殖 细胞凋亡 

分 类 号：R730.58[医药卫生—肿瘤]