农杆菌介导DR1372基因转化拟南芥的研究  

On Agrobacterium-mediated Transformation of DR1372 Gene into Arabidopsis thaliana

作　　者：张思维[1] 周文波[1] 张新[1] 陈翠娜[1] 代其林[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《绵阳师范学院学报》2013年第2期57-64,共8页Journal of Mianyang Teachers＇ College

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅(09ZA034);西南科技大学博士研究基金(11zx7104);农业部公益性行业科研专项(201103007)

摘　　要：耐辐射奇球菌(D.radiodurans,DR)在极端胁迫条件下能够继续生存,其耐辐射奇球菌基因组中(DRR1)拥有一个独特的极端环境抗性基因组而被广泛研究.DR1372基因就是在DR R1中克隆得到的一个基因,其蛋白序列存在一个Why功能域,此功能域可能参与了植物的抗旱过程.我们利用基因工程手段首先构建了植物DR1372-GV3103表达载体,然后利用花序浸染法成功将目的基因DR1372转入拟南芥中,最后对阳性植株进行盐胁迫,证实了DR1372基因在拟南芥中的表达明显改善了转基因植株的耐盐性.初步建立了农杆菌介导DR1372基因转化拟南芥体系,为后续DR1372基因的功能研究工作提供了理论基础.DR1372 is cloned from an extremely radiation resistant bacterium which named D. radiodurans. It contains a special domain called Why which may be involved in the mechanism of drought resistance of plants. In this study, the plant expression vector DR1372 -GV3103 was firstly constructed and DR1372 gene was inserted into Arabidopsis successfully by using the transgenie technique. Then, positive plants have got a salt stress and the results show that DR1372 gene improves the salt tolerance of Arabidopsis obviously. Thus, it not only contributes good conditions to later research on DR1372 gene, but also has great important application in plant gene engineering technology.

关 键 词：DR1372 载体构建 盐胁迫 拟南芥 

分 类 号：O175.12[理学—数学]