1个籼粳杂交F_1花药培养及其影响因素研究  

F_1 Anther Culture from Japonica/indica Hybrid Rice and its Affecting Factors

作　　者：苗立新[1] 李鑫[1] 李珣[1] 张丽丽[1] 孙杰[1] 刘中卓[1] 

机构地区：[1]辽宁省盐碱地利用研究所,辽宁盘锦124010

出　　处：《北方水稻》2013年第2期14-18,共5页North Rice

摘　　要：以籼粳杂交(沈农265/R99)F1及其亲本为试验材料,对低温预处理、不同基本培养基、基因型、不同激素配比等影响花药培养效果的因素进行了研究。结果表明:(1)不同低温处理时间沈农265/R99 F1的愈伤诱导率、绿苗分化率和花药培养力差异达到极显著水平,低温预处理7 d的花药培养效果最好。(2)3个基因型的愈伤诱导率、绿苗分化率以及花药培养力差异很大,沈农265/R99适合在SK3培养基上培养,沈农265适合在改良N6培养基上培养,R99适合在M8培养基上培养,培养基与基因型存在互作效应。(3)沈农265/R99F1花药诱导愈伤的最佳的激素配比为2,4-D(2 mg.L-1)、NAA(1mg.L-1)、KT(0.5 mg.L-1)。Effects of low temperature, different culture mediums, genotypes and different hormone mixed rate on anther culture were studied with japgnica/indica hybrid F1 （Shennong265/R99） as materials. The results showed that the differences of callus in- duction efficiency; green plantlet differentiation rate and anther culture ability were significant between different low temperature treatments, and low temperature treatment for 7 days had the best effects. The differences of callus induction efficiency, green plantlet differentiation rate and anther culture ability among 3 genotypes were significant and there was interaction between culture mediums and genotypes. The optimal hormone mixed rate was 2,4-D（2 mg. L-I）,NAA（1 mg. L-1） and KT（0.5 mg.L-1）.

关 键 词：籼粳杂交F1 愈伤诱导率 绿苗分化率 花药培养力 

分 类 号：S511.035.2[农业科学—作物学]