白介素-13基因多态性与哮喘的相关性研究  

Study on the correlation between asthma and IL-13 gene polymorphism

作　　者：濮海平 刘华 吕振华[2] 林荣军[3] 傅翠捧 孙晓梅 

机构地区：[1]401医院儿科,山东青岛266071 [2]青岛大学医学院附属医院分子生物实验室,山东青岛266001 [3]青岛大学医学院附属医院儿科

出　　处：《实用医药杂志》2013年第4期289-292,共4页Practical Journal of Medicine & Pharmacy

基　　金：青岛市科技发展项目(项目编号05-1-NS-76)

摘　　要：目的探讨白介素-13(IL-13)基因多态性与哮喘的相关性。方法用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)方法检测96例汉族哮喘儿童及96名汉族健康儿童IL-13基因内含子区+1923位点的基因型和等位基因频率,分析此位点单核苷酸多态性与哮喘的易感性、血浆总IgE(TIgE)、IL-13水平的相关性。结果+1923位点等位基因C、T频率在两组间分布具有显著性差异(P<0.01),等位基因T与哮喘关联,OR(T/C)=1.87,95%CI=1.25～2.80,P<0.01。两组基因型(TT、CT、CC)频率的分布亦有显著性差异(P<0.01),哮喘组中TT、CT基因型人群外周血IL-13及TIgE水平与同组及对照组CC基因组比较有显著性差异(P<0.01)。结论 IL-13基因+1923位点多态性是影响哮喘的重要候选基因,T等位基因与哮喘关联,并可能通过增强IL-13基因的表达影响血浆总IgE水平。Objective To discuss the association between asthma and IL-13 gene polymorphism. Methods A total of 192 children （the Han nationality） including 96 asthma and 96 control subjects were recruited. PCR- RFLP was used to detect the IL-13＋ 1923 SNPs. The differences of geneotype and allele frequency between the two groups were analyzed, so as to analyse the correlation of SNP with asthma susceptibility, total plasma IgE and IL-13 level. Results The allele frequencies of C, T at IL-13＋1923 site were evidently different between the two groups（P〈0.01）, indicating allele T correlates with asthma, OR（T/C）=1.89, 95%CI=1.25-2.80, P〈0.01. The difference of genotype frequencies（IT, CT, CC） of the two groups also was significant（P〈0.01）. The IL-13 and TIgE level of Tr, CT genomic persons was evidently different from other genomic persons in asthma group and that in healthy group （P〈0.01）. Conclusion IL-13 gene ＋1923 site polymorphism is of an important candidate genes that affect asthma, T allele is associated with asthma and may affect the total plasma IgE level by enhancing the expression of L-13.

关 键 词：白细胞介素-13 基因多态性 哮喘 

分 类 号：R562.25[医药卫生—呼吸系统]