银杏叶片生长过程中的叶绿素荧光动力学研究  

Chlorophyll Fluorescence Transient Kinetics of Ginkgo Leaves during Expansion

作　　者：杨贤松[1] 

机构地区：[1]蚌埠学院生物与食品工程系,安徽蚌埠233030

出　　处：《中药材》2013年第1期1-5,共5页Journal of Chinese Medicinal Materials

基　　金：安徽省自然科学基金项目(11040606M79);安徽省高等学校省级自然科学基金项目(KJ2010B108)

摘　　要：目的:为银杏的栽培管理提供理论依据。方法:以大田栽培的10年生银杏实生苗为试材,研究了自然条件下叶片生长过程中叶绿素荧光动力学特性。结果:Wk在12∶00～15∶00之间出现高峰,φEo及ψo在8∶00～12∶00之间逐渐下降。中午时段,ABS/RC、TRo/RC和DIo/RC明显增加,RC/CS下降。Fv/Fm和PIabs在1 d中先下降后上升在13∶00左右达到最低点,19∶00都能基本恢复到早7∶00的水平。结论:光抑制情况下PSⅡ反应中心供体侧和受体侧功能都受到暂时抑制。中午时的强光、高温伤害了PSⅡ的反应中心,使PSⅡ的光能转化效率下降,导致过剩激发能增加,光合作用原初反应过程受抑制。但这只是PSⅡ反应中心发生可逆失活,光合机构并未受到不可逆损伤。Objective：To provide the theoretical basis for cultivating ginkgo（Ginkgo biloba）.Methods：Changes of chlorophyll fluorescence transient kinetics in chloroplasts of 10-year-old seeded ginkgo leaves during expansion were studied in the field under natural environmental conditions.Results：Wk peaked between 12∶ 00~15∶ 00,φEo and ψo declined gradually between 8∶ 00~12∶ 00.ABS/RC,TRo/RC and DIo/RC increased,while RC/CS decreased at noon.PIabs and Fv/Fm declined and reached its lowest value at 13∶ 00,then increased,and the levels at 19∶ 00 could restore to the levels at 7∶ 00.Conclusion：The donor and acceptor sides of PS Ⅱ are temporarily inhibited.The reaction centers of PS Ⅱ are damaged,leading to the light energy transfer efficiency of PS Ⅱ decrease and excess excitation energy increase at noon.The damage of reaction centers of PS Ⅱ and inhibition of photosynthetic primary reaction are reversible inactivation not irreversible damaged.

关 键 词：银杏 叶片生长 叶绿素荧光动力学 

分 类 号：R282.2[医药卫生—中药学]