制山茱萸活性部位对D-半乳糖致小鼠肝损伤的保护作用  

Hepatoprotective Effects of Extracts from Processed Corni Fructus against D-galactose-induced Liver Injury in Mice

作　　者：姜泽群[1] 李育[1] 江励华[1] 顾海[1] 王明艳[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中药材》2013年第1期85-89,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(30772851);江苏省"六大人才高峰"第六批资助项目

摘　　要：目的:研究制山茱萸活性部位对D-半乳糖致小鼠肝损伤的保护作用。方法:采用D-半乳糖造成小鼠急性肝损伤模型,以血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)活性及肝脏丙二醛(MDA)含量作为考察指标,观察制山茱萸活性部位(醇沉多糖部位、正丁醇萃取部位、石油醚萃取部位)对小鼠急性肝损伤的保护作用。另取肝组织进行常规HE染色病理组织学切片,结合透射电镜观察肝细胞的凋亡情况。结果:生化分析结果表明,与正常对照组比较,模型组小鼠肝匀浆SOD活性显著降低,MDA含量及ALT、AST活性活性均显著升高(P<0.05);与模型组比较,三种制山茱萸活性部位干预组小鼠肝脏ALT、AST活性及MDA含量均显著降低,肝脏SOD活性显著升高(P<0.05)。肝脏病理形态学显示,模型组肝脏出现明显坏死和炎症,而制山茱萸活性部位干预组变性坏死明显减少,炎症细胞数减少,肝组织的损伤修复程度较好。电镜结果显示,模型组肝细胞损伤较为明显,小鼠肝脏细胞出现明显凋亡特征,而制山茱萸活性部位用药组未见凋亡。结论:制山茱萸多糖、正丁醇萃取部位、石油醚萃取部位均表现出保肝作用,提示三者均存在保肝活性成分,是制山茱萸保肝的物质基础所在,其保肝机制可能与抑制氧化应激及抗炎症反应有关。Objective：To study the hepatoprotective effects of extracts from processed Corni Fructus against D-galactose-induced liver injury in mice.Methods：Acute liver injury model was established by D-galactose.The activity of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,superoxide dismutase（SOD）and level of liver malondialdehyde（MDA）of serum was measured.Hematoxylin and Eosin（HE） staining of pathological section and transmission electron microscopic observation were used to measure the apoptosis of liver cells.Results：Compared with the normal control group,SOD activity was decreased,MDA level and ALT,AST activity was increased in the model group,and the differences were significant（P0.05）;While three kinds of cornel active sites showed significant improvement with increasing SOD activity and decreasing ALT,AST activity and MDA levels（P0.05）.Furthermore,model group appeared obvious necrosis inflammation,and apoptosis characteristics;While liver structural damage were improved significantly in cornel active site groups.Conclusion：Cornel polysaccharide extract,n-butanol extraction site and petroleum ether extraction sites all have hepatoprotective effects,suggesting that they are the active material of cornel product,and the mechanism may be related to the inhibition of oxidative stress and inflammatory response.

关 键 词：制山茱萸 活性部位 D-半乳糖 肝脏保护 

分 类 号：R285.5[医药卫生—中药学]