鼻咽清毒颗粒药物血清体外对鼻咽癌细胞增殖的影响  

Research on the Effect of Biyan Qingdu Granula Drug-containing Serum on Inhibition of Nasopharyngeal Carcinoma Cells Growth in vitro

作　　者：康敏[1] 王仁生[1] 刘文其[1] 唐安洲[1] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530021

出　　处：《中药材》2013年第1期89-92,共4页Journal of Chinese Medicinal Materials

基　　金：广西科技厅科技攻关项目(桂科攻0322024-1A)

摘　　要：目的:探讨鼻咽清毒颗粒药物血清(Biyan Qingdu Granula drug-containing serum,BQG-DS)对体外培养的鼻咽癌细胞株CNE1、CNE2、TWO3、C666-1细胞增殖的影响,并探讨其作用机制。方法:将SD大鼠随机分为3组,实验组(灌胃鼻咽清毒颗粒)、阳性对照组(灌胃环磷酰胺)、阴性对照组(灌胃生理盐水),动物给药后采血,提取制备含药血清。运用MTT法检测作用药物血清后细胞增殖的改变,并用流式细胞仪(FCM)测定细胞周期的改变,同时结合倒置显微镜,观察其诱导鼻咽癌细胞凋亡的作用。结果:鼻咽清毒颗粒可抑制CNE1、CNE2、TWO3、C666-1细胞的增殖,其作用具有时间和浓度依赖性;同时可诱导细胞凋亡,改变细胞周期分布,多数细胞阻滞于G1期。结论:鼻咽清毒颗粒可诱导鼻咽癌细胞凋亡,改变细胞周期分布,从而抑制细胞增殖。Objective：To investigate the effect of Biyan Qingdu Granula drug-containing serum（BQG-DS） on cell growth and apoptosis in nasopharyngeal carcinoma cell lines CNE1,CNE2,TWO3,C666-1,and explore the antineoplastic mechanism of Biyan Qingdu Granula.Methods：SD rats were randomly divided into three groups：experimental（Biyan Qingdu Granula）group,positive control（cytoxan） group and negative control group.After administration of drug,the serum was collected from the treated animals.MTT assay was used to examine the effect of BQG-DS on the proliferation of CNE1,CNE2,TWO3,C666-1 cell,and flow cytometry was used to observe the cell cycle distribution.Apoptosis of CNE1,CNE2,TWO3,C666-1 cell was further investigated by inverted microscope.Results：BQG-DS inhibited the proliferation of CNE1,CNE2,TWO3,C666-1 cell and the effects were in a time-and concentration-dependent manner.BQG-DS could also induce apoptosis while the G1 phase was arrested.Conclusion：BQG-DS inhibits proliferation of nasopharyngeal carcinoma cells via induction of apoptosis and arrest of cell cycle.

关 键 词：鼻咽癌 鼻咽清毒颗粒 药物血清 增殖 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]