黄葵胶囊中5种黄酮类成分的含量测定及其特征图谱研究  

Simultaneous Determination of Five Flavonoids and Specific Chromatograms Analysis of HuangKui Capsule

作　　者：刘霖[1] 唐海涛[2] 刘汉清[1] 王进[1] 张平[2] 唐云[1] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏苏中药业集团股份有限公司,江苏姜堰225500

出　　处：《中药材》2013年第1期132-136,共5页Journal of Chinese Medicinal Materials

基　　金：江苏省重点科研成果产业化项目(BA2012131)

摘　　要：目的:建立同时测定黄葵胶囊中5种黄酮类成分含量的方法,并对其特征图谱进行研究。方法:采用HPLC法,色谱条件为:Thermo scientific Hypersil GOLD(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.2%磷酸水溶液为流动相进行梯度洗脱,流速0.8 mL/min,波长360 nm,柱温30℃。结果:5种黄酮成分(芦丁、金丝桃苷、异槲皮苷、杨梅素、槲皮素)具有良好的分离度,相关系数均为0.9999,平均回收率为98.46%～100.33%,并用此法建立了黄葵胶囊的特征图谱,包含15个共有峰,采用对照品指认了其中5个峰,分析测定了10批黄葵胶囊的特征图谱,相似度均在0.95以上。结论:该文所建立的含量测定及特征图谱分析方法特征性和专属性强,且快速、简便、可靠,可用于全面控制该制剂的质量。Objective：To Set up a method for determining the contents of the five flavonoids simultaneously in the HuangKui capsule and analyze their specific chromatograms.Methods：HPLC method was used.The analytical column was Thermo scientific Hypersil GOLD（250 mm×4.6 mm,5 μm）.The mobile phase was composed of acetonitrile（A）and 0.2% orthophosphoric acid（B）with gradient elution.The flow rate was 0.8 mL/min and detection wavelength was 360 nm.The column temperature was maintained at 30℃.Results：Contents of the five flavonoids（Rutin,Hyperoside,Isoquercitrin,Myricetin,Quercitrin） had good resolution with the correlation coefficients exceed 0.9999 and the average percent recovery lied in 98.46% to 100.33%.The chromatograms of the HuangKui capsule shared 15 common peaks in which 5 of them were recognized by the reference standard.Chromatograms of 10 lots of HuangKui capsule were analyzed with the similarities over 0.95.Conclusion ：The proposed method of contents determination and chromatogram analysis has strong characteristic and specificity.This method is fast,easy and reliable,and can be applied for quality control of the preparation.

关 键 词：黄葵胶囊 HPLC 黄酮 含量测定 特征图谱 

分 类 号：R284.1[医药卫生—中药学]