大豆粒形性状QTL的精细定位  

Fine Mapping of Quantitative Trait Loci for Seed Shape Traits in Soybean

作　　者：牛远[1] 谢芳腾[1] 布素红[1] 谢尚潜[1] 韩世凤[1] 耿青[1] 刘兵[1] 章元明[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《作物学报》2013年第4期609-616,共8页Acta Agronomica Sinica

基　　金：教育部新世纪优秀人才支持计划项目(NECT-05-0489);江苏省自然科学基金项目(BK2008335);中央高校基本科研业务费专项资金创新团队项目(KYT201002)资助

摘　　要：在溧水中子黄豆×南农493-1衍生的504个F2:6家系中选择Satt331～Satt592目标区间7个杂合单株和168个重组单株,衍生成356株RHL-F2个体(群体Ⅰ)和168个重组体家系(群体Ⅱ)。群体Ⅱ来自142个F2:6家系,若每个F2:6家系只保留1个重组家系则构成群体Ⅲ。采用lasso和复合区间作图(CIM)法检测3个群体粒形性状2种指标的QTL。结果表明,lasso法检测到的粒长关联标记是O19和S21/Satt331,而CIM检测到的QTL区间是S21～S22和O23～O19;lasso法检测到的粒宽关联标记是O19/O21,而CIM检测到的QTL区间是O23～O19/O19～O21;长宽比与S21～S22关联是由于粒长QTL引起的,与O23～O19/O19～O21关联是由于粒长和粒宽QTL引起的。将原Satt331～Satt592目标区间的粒长QTL剖分为与标记S21～S22和O23～O19/O19～O21关联的2个多效性QTL。根据大豆基因注释数据库,Glyma10g35240和Glyma10g34980可能是控制粒形性状发育的候选基因。Based on the target interval region of SSR markers Satt331-Satt592, we constructed 356 RHL-F2 individuals （popula- tion I） and 168 recombinant families （population II） derived from 504 F2：6 families of the direct and reciprocal crosses of Lishuizhongzihuang with Nannong 493-1 to fine map quantitative traits loci （QTLs） for seed length （SL）, width （SW）, and length-to-width （SLW） ratio in soybean using composite interval mapping （CIM） and lasso approaches. Among the 168 recombi- nant families, only one recombinant individual was selected from a same F2：6 family so that 142 recombinant families were constructed as population III. Response variable for QTL analysis was as original observation, and the value corrected by the associated marker information. As a result, markers associated with SL were O 19 and S21/Satt331 from lasso method and $21-$22 and O23~O19 from CIM method; marker associated with SW was O19/O21 from lasso method and O23-O19/O19-O21 from CIM method; and the QTL associated with markers $21-$22 for SLW was derived from QTL for SL, and the QTL with markers O23-O19/O19-O21 for SLW was derived from QTLs for SL and SW. This indicates that QTL associated with markers Satt331-Satt592 in our study was further partitioned into two pleiotropic QTLs, which were associated with markers S21-S22 and markers O23-O19/O19-021. According to the annotation project database, Glyma10g35240 and Glyma10g34980 might be candidate genes for seed shape traits.

关 键 词：大豆 粒形 数量性状基因座 精细定位 候选基因 

分 类 号：S565.1[农业科学—作物学]