小鼠NMDA受体NR2B亚基短发夹RNA干扰真核表达载体的构建与鉴定  

Construction and identification of N-methyl-D-aspartate receptor NR2B subunit-short hairpin RNA eukaryotic expression vector

作　　者：章饶香[1] 

机构地区：[1]中南大学湘雅三医院麻醉科,湖南长沙410013

出　　处：《现代医药卫生》2013年第7期1008-1009,共2页Journal of Modern Medicine & Health

摘　　要：目的设计并构建小鼠N-甲基-D-天冬氨酸(NMDA)受体2B亚基(NR2B)短发卡RNA干扰真核表达载体并鉴定。方法根据NMDA受体NR2B mRNA编码序列设计并合成针对NR2B的特异性RNA干涉片段,将其克隆入pYr-1.1质粒载体,构建NR2B短发夹RNA(shRNA)真核表达载体pYr-1.1-GRIN2B-shRNA,并对其进行酶切和测序鉴定。结果酶切和测序结果均证明NMDA受体NR2B shRNA已经插入质粒载体pYr-1.1。结论 NMDA受体NR2B shRNA真核表达载体pYr-1.1-GRIN2B-shRNA构建成功。Objective To design and construct short hairpin RNA（shRNA） eukaryotic expression plasmids targeting the gene of N-methyl-D-aspartate （NMDA） receptor NR2B subunit and its identification. Methods According to NMDA receptor NR2B subunit mRNA coding sequence ,the specific RNA interference（RNAi） fragments targeting the gene of NR2B subunit were designed and synthesized ,which were cloned into pYr-l.1 plasmid vector, and the shRNA eukaryotic expression vector pYr-I.1- GRIN2B-shRNA targeting the gene of NR2B subunit was constructed,and then identified by restriction analysis and sequencing test. Results The results of restriction analysis and sequencing test showed that NMDA receptor NR2B subunit shRNA.was suc- cessfully inserted into the plasmid vector pYr-1.1. Conclusion The pYr-1.1-GRIN2B-shRNA expression vectors is constructed successfully.

关 键 词：小鼠 RNA干扰 NMDA受体NR2B亚基 shRNA干扰载体 

分 类 号：R595.5[医药卫生—内科学]