SAH后下丘脑中5-羟色胺、去甲肾上腺素水平改变及地塞米松的作用  

Levels of 5-hydroxytryptamine and noradrenaline in rat hypothalamus after subarachnoid hemorrhage and effects of dexamethasone

作　　者：刘艳[1] 熊丹[1] 张小洁[1] 钱聪[2] 信照亮[1,3] 

机构地区：[1]温州医学院,325035 [2]浙江大学医学院附属邵逸夫医院神经外科 [3]浙江医学高等专科学校

出　　处：《浙江医学》2013年第6期418-420,440,共4页Zhejiang Medical Journal

基　　金：国家自然科学基金项目(30370497);浙江省社会发展项目(2007C33039)

摘　　要：目的观察大鼠实验性蛛网膜下腔出血(subarachnoid hemorrhage,SAH)后下丘脑组织匀浆5-羟色胺(5-hydroxytryptamine,5-HT)、去甲肾上腺素(Noradrenaline,NA)水平随时间变化情况,以及地塞米松(dexamethasone,DXM)不同给药途径对两者水平的影响。方法实验大鼠随机分为空白对照组、SAH组、腹腔给药组和脑池内给药组,按时间分为3、7、14、21d 4个亚组,采用高效液相色谱法(HPLC)测定下丘脑组织中5-HT和NA的含量。结果 5-HT水平在SAH后3d开始上升,7d达到高峰,14d仍维持在较高水平,21d下降到接近3d时的水平;NA的水平从第3天开始上升,第7、14、21天,水平呈逐渐升高的改变,与5-HT变化的时间模式不一致。DXM治疗组中5-HT水平均较SAH组明显降低(均P<0.05),且两种用药途径间无明显差异(P>0.05);DXM治疗对NA水平影响不大(P>0.05)。结论 SAH发生后,可导致下丘脑组织中5-HT和NA水平升高,可能与SAH后睡眠/觉醒异常的发生相关,但两者变化的时间模式不同。DXM可降低SAH后下丘脑组织中5-HT水平,可能是其促进清醒的机制之一。DXM脑池内给药是一种可选择的用药途径。Objective To investigate the levels of 5-hydroxytryptamine （5-HT） and norepinephrine （NA） in rat hypothalamus after subarachnoid hemorrhage （SAH）, and the effect of dexamethasone （DXM） on 5-HT and NA. Methods The rats were randomly divided into 4 groups： the blank, SAH control, SAH treated by intracisternal DXM and SAH treated with intraperitoneal DXM. The levels of 5-HT and NA were measured by high performance liquid chromatograph. Results The 5-HT in hypothalamus tissue began to rise in 3d, reached to peak in 7cl, remained at high level in 14d and went down to d3 level in 21d, which was consistent with the timing of delayed cerebral ischemic. The NA in hypothalamus tissue began to rise in 3d and kept rising in 7, 14 and 21d, which was not consistent with the mode of 5-HT. The levels of 5-HT were decreased after DXM treatment and there was no significant difference between intracisternal and intraperitoneal administration. There were no changes of NA levels after DXM treatment. Conclusion 5-HT and NA levels in hypothalamus are increased after SAH, which may be involved in abnormality of sleep/wakefulness, but the time modes of 5-HT and NA are different. DXM may promote wakefulness by lowering 5-HT level in hypothalamus after SAH, and DXM can be administrated intracisternally as an alternative way.

关 键 词：蛛网膜下腔出血5一羟色胺去甲肾上腺素地塞米松 

分 类 号：R965[医药卫生—药理学]