谷氨酰胺对慢性阻塞性肺疾病患者外周血单个核细胞中核因子-κB及IL-6的影响  

Effect of glutamine on NF-κB and IL-6 expression in PBMC of patients with chronic obstructive pulmonary disease

作　　者：居建刚[1] 黄美健[1] 周凌燕[1] 汤晓燕[1] 冷报浪[1] 任娥[1] 

机构地区：[1]安徽医科大学杭州临床学院(杭州市第三人民医院)呼吸内科,杭州310009

出　　处：《浙江医学》2013年第6期428-430,437,共4页Zhejiang Medical Journal

基　　金：杭州市卫生局基金项目(2011B009)

摘　　要：目的通过观察谷氨酰胺(GIn)对慢性阻塞性肺疾病(COPD)患者外周血单个核细胞(PBMC)中NF-κB活性和IL-6水平的影响,探讨GIn在COPD患者治疗中的抗炎作用。方法收集30例COPD患者在COPD急性加重期(AECOPD)和COPD稳定期(SCOPD)的外周血PBMC各30例,各分为GIn组细胞培养时加入浓度为8 mmol/L的GIn和空白对照组,并以15例健康体检者作为对照组。采用RT-PCR法测定5组外周血PBMC中NF-κBp65、IL-6基因的表达。结果健康对照组NF-κBp65、IL-6表达水平低于AECOPD空白对照组(均P<0.01),与SCOPD空白对照组比较(均P<0.05)。AECOPD GIn组与健康对照组NF-κBp65、IL-6比较有统计学意义(均P<0.05),而SCOPD GIn组NF-κBp65、IL-6与健康对照组比较无明显差异(均P>0.05)。AECOPD GIn组与空白对照组比较NF-κBp65、IL-6的表达明显降低(均P<0.05)。SCOPD空白对照组NF-κBp65、IL-6与GIn组比较表达有统计学意义(均P<0.05)。结论 GIn可抑制COPD患者炎性转录因子NF-κB活性,并下调IL-6的表达水平。Objective To determine the effects of glutamine on the nuclear factor kappa B （NF-κB） activity and IL-6 expression in peripheral blood mononuclear celIs（PBMC） of patients with chronic obstructive pulmonary disease（COPD）. Methods PBMCs were collected from COPD patients with acute exacerbation （AECOPD group, n=30） , stable COPD patients （SCOPD group, n=30） and 15 health controls. Two COPD groups were further divided into 4 subgroups： blank control and glutamine （Gin） groups with 15 patients in each. The levels of NF- κBp65 and IL-6 gene expression in PBMCs were detected by real-time PCR. Results The expression of NF-κBp65 and IL-6 in blank control subgroups of AECOPD and SCOPD patients was higher than that in control group （P〈0.01）. The expression of NF-κ Bp65 and IL-6 in AECOPD Gin subgroup was still higher than that in control group （P〈0.05）, while there was no significant difference between control group and SCOPD Gin subgroup （P 〉0.05）; Compared with AECOPD and SCOPD blank subgroups, the expression of NF- κBp65 and IL-6 were decreased in AECOPD and SCOPD Gin subgroups （P〈0.05）. Conclusion Glutamine can inhibit the activation of NF-κB and down-regulate IL-6 level in PBMCs of patients with chronic obstructive pulmonary disease.

关 键 词：慢性阻塞性肺疾病 谷氨酰胺 单个核细胞 核因子-ΚB 白介素-6 

分 类 号：R563.9[医药卫生—呼吸系统]