长期培养外周血单个核细胞的简易分离纯化方法  

Simple isolating and purifying method for long-term cultured peripheral blood mononuclear cells

作　　者：罗利琼[1] 陈诚华[1] 张军[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006

出　　处：《新乡医学院学报》2013年第4期249-250,共2页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:31100198)

摘　　要：目的对长期培养的外周血单个核细胞(PBMC)进行纯化,为后续研究提供方便。方法用聚蔗糖-泛影葡胺密度梯度离心法对长期培养的PBMC进行再次分离和纯化。结果用聚蔗糖-泛影葡胺密度梯度离心法再次处理长期培养的PBMC,可以得到纯度较高的PBMC。结论聚蔗糖-泛影葡胺密度梯度离心法是纯化长期培养的PB-MC的一种简便、快捷、成本低的方法之一。Objective To purify the long-term cultured peripheral blood mononuclear cells（PBMC）and provide convenience for the subsequent research.Methods The long-term cultured PBMC were isolated and purified by Ficoll-Hypaque density gradient centrifugation.Results After the above centrifugation,a higher purity of PBMC can be easily obtained.Conclusion The Ficoll-Hypaque density gradient centrifugation is a simple,fast,low cost method for purifying long-term cultured PBMC.

关 键 词：外周血单个核细胞 聚蔗糖-泛影葡胺密度梯度离心法 细胞培养 

分 类 号：Q2-33[生物学—细胞生物学]