小麦种子萌发时期胚差异蛋白表达分析  

Analysis of Differentially Expressed Proteins of Embryo During Seed Germination in Wheat

作　　者：刘向标[1] 段江燕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041000

出　　处：《生物技术通报》2013年第3期60-64,共5页Biotechnology Bulletin

基　　金：山西省自然科学基金项目(2009011044-2)

摘　　要：为了解小麦种子在萌发时期胚蛋白质表达情况,通过双向电泳技术,对"晋麦-47"小麦种子未萌发和萌发3 h、16 h和25 h的胚进行蛋白质分离。结果发现,"晋麦-47"小麦种子未萌发和萌发3 h、16 h和15 h的胚在PDQuest图像分析软件可识别的蛋白点分别有127、131、135和141个,其中表达量变化2.5倍以上的蛋白点有17个。选取表达量在2.5倍以上的9个差异蛋白点进行质谱分析,初步鉴定出4个蛋白质,分别是核苷二磷酸激酶Ⅰ、17.5 kD热休克蛋白、ATP合酶和LEA蛋白27。In order to investigate the embryo protein expression of the wheat seeds germination, tow-dimensionsl gel electrophoresis （ 2-DE ） technology was used to separated proteins from ungerminated seed and embryo buds of germinated seed at specific stages of 3h, 16h, 25h in wheat ＂Jinmai-47＂ . The results showed that on the 2-DE gels PDQuest image software detected about 127, 131, 135, 141 protein spots, of which 17 spots show more than 2.5-fold changes in abundance among ungerminated seed and embryo buds of germinated seed at specific stages of 3 h, 16 h, 25 h in wheat ＂Jinmai-47＂ . These 9 of 17 protein spots treated by mass spectrometry analysis, of which 4 differential proteins were elementarily identified as nucleoside two phosphoglycerate kinase Ⅰ , 17.5 kD heat shock protein, ATP synthase and LEA protein 27.

关 键 词：小麦 胚 双向电泳 质谱技术 

分 类 号：S512.1[农业科学—作物学]