美洲黑杨与大青杨杂种无性系分子鉴定  

Molecular Identification of Hybrid Clones Between Populus deltoides Bartr. and P. ussuriensis Kom.

作　　者：江锡兵[1,2] 张志毅[2] 龚榜初[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]北京林业大学林木育种国家工程实验室林木花卉遗传育种教育部重点实验室,北京100083

出　　处：《生物技术通报》2013年第3期102-106,共5页Biotechnology Bulletin

基　　金：中国林业科学研究院亚林所基本科研业务费专项资金项目(RISF61253);林业公益性行业科研专项(201004009)

摘　　要：采用AFLP标记技术,利用EcoR I+2/Mse I+3引物组合对美洲黑杨与大青杨12个杂种无性系进行分子鉴定,结果表明,从32对引物组合中筛选出10对扩增条带较多的引物组合,扩增得到总条带数在7-52条之间,多态性条带数在2-28条之间,条带多态性比例分布范围为23.5%-41.6%,多态性条带数和多态性比例均较低,表明各个参试样品之间的亲缘关系较近;UPGMA聚类分析结果与12个无性系的系谱关系一致,表明亲本相同的无性系具有较高的相似系数;利用6个引物组合的9条多态性条带绘制了12个无性系的指纹图谱,为美洲黑杨与大青杨杂种无性系鉴定以及品种保护提供依据。Molecular identification of 12 hybrid clones were carried out using primer combinations of EeoR I ＋ 2 / Mse 1 ＋ 3 by adopting AFLP marker technology. The results were as followeds： 10 primer combinations with more amplification bands were selected from 32 primer combinations, the total number of bands were between 7 and 52, number of polymorphism bands were fi＇om 2 to 28, polymorpbism level of those primer combinations were from 23.5% to 41.6%, and lower level of polymorphism bands and proportion showed thai the relationships of the samples were closed. The results of UPGMA cluster analysis and pedigree relations of 12 clones were the same, which indicated that clones with the same parents had higher genetic similarity coefficient. The fingerprinting map of 12 hybrid clones were draw using 9 polymorphism bands from 6 primer combinations, which would provide bases of identification and protection for hybrid clones.

关 键 词：杨树 杂种 AFLP 分子鉴定 指纹图谱 

分 类 号：S792.11[农业科学—林木遗传育种]