一株耐盐好氧反硝化细菌的分离筛选及鉴定  

Screening and Primary Identification of an Aerobic Denitrifier Isolate with Salt Tolerance

作　　者：石小彤[1] 李彦芹[1] 邢国伟[1] 康现江[1] 

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《生物技术通报》2013年第3期175-180,共6页Biotechnology Bulletin

基　　金：海洋公益性行业科研专项经费子课题(201305001-4)

摘　　要：对对虾养殖池底泥进行富集分离,从4株反硝化细菌中筛选出一株高效的好氧反硝化菌株F1。该菌能在好氧条件下,40 h内高效地将硝酸盐浓度由442.28 mg/L降至58.28 mg/L,亚硝酸盐浓度由32.79 mg/L降至0.98 mg/L,反硝化作用主要发生在对数生长期。经菌落形态学特征、生理生化反应及16S rDNA分子鉴定,初步鉴定为盐单胞菌。Selective enrichment culture method under oxygen conditions was used for screening bacteria, the 4 strains which have capability of aerobic denitrification was isolated from a shrimp pond. A strong denitrifying effect bacteria was selected from them （ F1 ） . The bacterium reduced the concentrations of nitrate nitrogen rapidly from 442.25 mg/L to 58.25 mg/L, nitrite nitrogen from 32.79 mg/Lto 0.98 mg/L with 40 h in aerobic conditions. It identified as Halomonas sp. based on its biochemical and morphological characters. Phylogenetic analysis of 16S rDNA sequences indicated that strain F1 was most closely related to Halomonas alimentaria. The denitrification mainly occurs in the logarithmic grmwh phase.

关 键 词：好氧反硝化细菌 反硝化效率 盐单胞菌 耐盐 

分 类 号：X703[环境科学与工程—环境工程] X172