鱼类核移植与重编程  

Nuclear transfer and reprogramming in fish

作　　者：王学耕[1] 朱作言[1] 孙永华[2] 赵珏[1] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉430072

出　　处：《遗传》2013年第4期433-440,共8页Hereditas（Beijing)

基　　金：斑马鱼细胞核移植发育再程序化研究项目(编号:30950001)资助

摘　　要：鱼类核移植是动物克隆研究的一个重要领域,我国学者在上世纪60年代首创了鱼类的核移植研究。以斑马鱼为模式动物,进行核移植与再程序化研究具有独特的优势。文章总结了鱼类细胞核移植研究的历史、斑马鱼核移植研究概况、以及影响核移植胚胎发育的因素,特别是核移植胚胎基因组的表观遗传修饰,如基因组DNA甲基化及组蛋白乙酰化和甲基化等的研究,将有助于完善克隆技术并提高克隆的成功率,推动克隆技术的广泛开展和应用。As an important sub-field in the study of animal cloning, fish nuclear transfer was first established in the early 1960s by Chinese embryologists. Due to its advantages, zebrafish has become a unique animal model to study the mystery of reprogramming in nuclear transfer. This article summarizes the history and current situation in fish nuclear transfer tech- nology and discusses the factors that may influence the development of the cloned embryos. A comprehensive understand- ing of the mechanism for epigenetic modification following nuclear transfer, such as genomic DNA methylation and histone acetylation and/or methylation, will likely increase the success rate and eventually lead to the future freedom of cloning technique.

关 键 词：核移植 斑马鱼 再程序化 表观遗传修饰 

分 类 号：Q813[生物学—生物工程]