PCR-SSP法检测吉林省汉族人群血小板抗原1～6系统研究  

Study on genotyping of HPA1～6 system by PCR-SSP in Jilin Han population

作　　者：张冬霞[1] 黄氏敏 于红[1] 李颖[1] 史立英[1] 刘冰[2] 

机构地区：[1]吉林省肿瘤医院输血科,长春130012 [2]吉林大学第一医院输血科

出　　处：《临床血液学杂志（输血与检验）》2013年第2期240-242,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：吉林省卫生厅课题(No:2009z029)

摘　　要：目的:应用PCR-SSP法对吉林省汉族人群人血小板同种抗原系统(HPA)1～6进行基因及其多态性分布特征研究。方法:用DNA试剂盒提取外周血标本中DNA,通过特异性引物(PCR-SSP)扩增HPA等位基因。检测200名HPA1～6抗原系统共12个抗原的基因分型。结果:200名无偿献血并无血缘关系的吉林省汉族人群HPA基因频率为:HPA-1a0.9900、1b0.0100;2a0.9300、2b0.0700;3a0.5575、3b0.4425;4a1.0000、4b0.0000;5a0.9900、5b0.0100;6a0.9875、6b0.0125。结论:吉林地区血小板志愿捐献者HPA1～6抗原系统的基因频率符合Hardy-Weinberg遗传定律,与其他地区和国家的同类资料相比显示出种族和地域性差异,由此建立起了吉林地区已知基因型的血小板捐献者资料库。Objective：To analyze the gene polymorphism distribution characteristics of human platelet antigen （HPA）1-6 using polymerase chain reaction with sequence specific primers （PCR-SSP） in Jilin Han population. Metbod：DNA was extracted from peripheral blood samples from 200 Han donors in Jilin Province by DNA kit and PCR-SSP was introduced to analyze the genotypes of HPA 1-6. Result： HPA allele frequencies of 200 Han donors in Jilin Province were HPA-la 0. 9900,1b 0. 0100;2a 0. 9300,2b 0. 0700;3a 0. 5575,3b 0. 4425;4a 1. 0000,4b 0. 0000 ; 5a 0. 9900,5b 0. 0100 ; 6a 0. 9875,6b 0. 0125. Conclusion; HPA allele frequencies of Han population in jilin province fit Hardy-Weinberg equilibrium, and compared with other regions and countries, they had race and regional differences. Thus a database of platelet donors with known HPA genotype was established in Jilin province.

关 键 词：PCR-SSP法 人血小板同种抗原 基因频率 

分 类 号：R596[医药卫生—内科学]