Bmp8a和Bmp8b基因在体外培养小鼠卵巢颗粒细胞中的表达  

Expressions of Bmp8a and Bmp8b in cultured mouse granulosa cells

作　　者：王建起[1] Selvaraj J.NIMAL 曹文广[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国农业大学学报》2013年第3期108-114,共7页Journal of China Agricultural University

基　　金：国家转基因重大专项资助(2011ZX08008-003)

摘　　要：为研究体外培养的小鼠卵巢颗粒细胞Bmp8a和Bmp8b基因的表达情况,用10IU PMSG皮下注射21～23日龄健康雌性ICR小白鼠,48h后刺破卵泡获取颗粒细胞,置入10%FBS的DMEM/F12中体外培养5d,用Trizol提取总RNA,RT-PCR验证后切取目的 DNA片段进行测序。RT-PCR测序结果表明体外培养的小鼠卵巢颗粒细胞可扩增BMP-8atranscript variant 2和BMP-8b的mRNA序列,不扩增BMP-8atranscript variant 1的mRNA序列。结果显示体外培养的小鼠卵巢颗粒细胞可表达BMP-8atranscript variant 2和Bmp8b基因。This study was to analyze the expressions of Bmp8a and Bmp8b in cultured mouse granulosa cells,for further understanding of the mechanism of follicular development.Twenty-one to twenty-three day-old female mice were hypodermically injected with 10IU of PMSG.After 48 hours,the bilateral ovaries were removed and the granulosa cells were collected by piercing the ovarian follicles.The granulosa cells were seeded in cell culture plates in DMEM/F12 supplemented with 10% FBS.After 5 days,the cells were collected from the wells and total RNA was extracted.The expressions of Bmp8a and Bmp8b were analyzed by RT-PCR and confirmed by sequencing.RT-PCR sequencing results showed that in vitro cultured mouse granulosa cells generated BMP-8a transcript variant 2 and BMP-8b mRNA.And BMP-8a transcript variant 1 mRNA was found to be absent.Our results showed that in vitro cultured mouse granulosa cells could express BMP-8a transcript variant 2 and Bmp8b.

关 键 词：小鼠 卵巢颗粒细胞 Bmp8a Bmp8b 

分 类 号：Q2[生物学—细胞生物学]