人羊膜间充质干细胞静脉移植后在心肌梗死大鼠体内的定植及分化  

Human amnion-derived mesenchymal stem cells engraftment and differentiate in rats with acute myocardial infarction

作　　者：王钰莹[1] 方宁[1] 陈代雄[1] 余丽梅[1] 刘祖林[1] 万雪[1] 

机构地区：[1]遵义医学院附属医院贵州省细胞工程重点实验室,贵州遵义563003

出　　处：《中国老年学杂志》2013年第7期1583-1586,共4页Chinese Journal of Gerontology

基　　金：贵州省科技厅攻关项目(黔科合SZ字〔2009〕3017);"重大新药创制"同科技重大专项课题(2009ZX09503-025)

摘　　要：目的观察大鼠心肌梗死(MI)后经静脉移植的人羊膜间充质干细胞(hAD-MSCs)在体内的定植、分化及对心功能的影响。方法SD大鼠随机分为hAD-MSCs移植组,模型组和假手术组各12只。分离hAD-MSCs用流式细胞仪和免疫组化染色鉴定其表型特征。制模后1 w经舌下静脉移植Brdu标记的hAD-MSCs,于移植后6 w将大鼠心脏取出并切片行组织病理学、免疫组织化学、双重免疫荧光染色等检测,以观察植入的细胞在MI区的定植、分化,在肝、脾中的分布情况。结果分离的hAD-MSCs具有典型的间充质干细胞表型特征,高表达CD44和波形蛋白;静脉移植后6 w,心脏MI区、肺脏和脾脏中均可见Brdu阳性细胞,但在肝脏中未见阳性细胞,移植的hAD-MSCs表达心肌特异蛋白连接蛋白-43和α-辅肌动蛋白。移植后6 w,移植组大鼠心功能没有进一步恶化。结论 hAD-MSCs经静脉移植能够定植于大鼠MI区域,可分化为心肌细胞,并改善心功能。Objective To observe the distribution and differentiation of hAD-MSCs injected intravenously and the feasibility of it used to repair the infracted heart. Methods SD rats were randomly assigned into hAD-MSCs transplanted, model and sham operation groups （ 12 rats each group）. The hAD-MSCs were isolated from human amnion and then its phenotype was identified by flow cytometry and immu- nohistochemical staining, hAD-MSCs were injected into the subglossal vein of the SD rats 7 days after myocardial infarction （MI） was created and the organs such as the heart, liver and spleen were harvested at 6 weeks after transplantation. In order to assess the survival and differen- tiation of hAD-MSCs posttransplantation in the MI zone, as well as the influence on cardiac function. Results The isolated hAD-MSCs had the typical features of mesenchymal stem cells （ MSCs）, high expression of CD44, CD73, CD90, CD105 and vimentin. After injection through the subglossal vein, the hAD-MSCs passed through the capillary of the lung, and then distributed into the heart, lung and spleen, but seldom in the liver. At 6 weeks after hAD-MSCs transplantation, cell engraftments expressed cardiac-specific protein connexin-43 and cL- actinin. The left ventricle function did not exacerbate after injection of hAD-MSCs compared with that in control group. Conclusions hAD- MSCs injected intravenously can distribute into myocardium, lung and spleen. The migration of injected cells delays the left ventricle remode- ling after MI.

关 键 词：人羊膜间充质干细胞 心肌梗死 细胞移植 

分 类 号：R39[医药卫生—基础医学]