过氧化物酶体增殖物激活受体-γ辅激活因子-lα对氧-糖剥夺神经元存活率及活性氧水平影响  

Effect of PGC-1α on the survival rate and reactive oxygen species of injured neurons induced by oxygen-glucose deprivation

作　　者：韩勇[1] 沙杜鹃[2] 顾双双[1] 王路娜[1] 李瑾[2] 李启明[2] 张均[1,2] 

机构地区：[1]南京医科大学鼓楼临床医学院急诊科 [2]南京大学附属南京鼓楼医院急诊科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2013年第3期323-326,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市医学科技发展项目(YKK11090)

摘　　要：目的:探讨过氧化物酶体增殖物激活受体-γ辅激活因子-lα(PGC-1α)对氧糖剥夺神经元的保护作用及机制。方法:原代培养胚胎小鼠大脑皮层神经元7 d,细胞随机分为4组:正常组、氧糖剥夺组(OGD组)、OGD+PGC-1α过表达质粒组(过表达组)、OGD+空载质粒组(空载组)。过表达组与空载组分别于OGD处理前予以PGC-1α过表达质粒和空载质粒预处理。采用蛋白印迹技术检测PGC-1α表达,噻唑蓝比色分析(MTT)法检测神经细胞存活率,流式细胞仪检测活性氧(ROS)含量。结果:OGD组较正常组神经元存活率明显下降(P<0.01);予以PGC-1α过表达质粒预处理后,PGC-1α蛋白水平明显上调,其神经元存活率升高,与OGD组及空载组比较具有显著差异性(P<0.01)。OGD组神经元ROS含量明显高于正常组(P<0.01);过表达组ROS水平降低,与OGD组及空载组ROS含量比较其差异具有统计学意义(P<0.01)。结论:PGC-1α明显降低氧糖剥夺损伤神经元ROS含量,提高其生存率,发挥其神经保护作用。Objective：To explore the protective effects and mechanism of Peroxisome proliferative activated receptor-~, activation of auxiliary factor 1 Alpha （PGC-1α） on injured neurons induced by oxygen-glucose deprivation （OGD） in embryonic mice.Methods： Cortical neurons cultured for 7 days were randomly divided into four groups：normal control group,oxygen-glucose deprivation group （OGD）;OGD ＋ PGC-1α overexpression plasmid group （overexpression group） and OGD＋no-load plasmid group （no-load group）. Overexpression group and the no-load group pretreatment with PGC-1α overexpression plasmid and no-load plasmid respectively before OGD treatment. 24 hours after OGD, cell survival rate was detected by MTT （Methyhhiazolyldiphenyl-tetrazolium bromide）,observe the expression of PGC-1α was using Western blot technology,and the flow cytometry was used to monitor the content of Reactive Oxygen Species （ROS）. Results：Compared with the normal control group,the cell survival rate in OGD group decreased（P 〈 0.01 ）,the survival rate was increased in overexpression group due to pretreatment of PGC-1α overexpression plasmid,which was compared with OGD group and no-load group with a significant difference （P 〈 0.01 ）. The ROS of OGD groups were significantly higher than that of the normal group （P 〈 0.01 ）;the content of ROS was decreased in overexpression group, and was significantly lower than that of the OGD group and no-load group （P 〈 0.01）. Conclusion：The study indicated that PGC-1α can significantly reduce the ROS content of oxygen-glucose deprivation neurons and improve its survival rate, to protect the neurons.

关 键 词：过氧化物酶体增殖物激活受体-Γ 辅激活因子-lα 神经元 氧化应激 氧糖剥夺 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学]