落叶松毛虫2种解毒酶的体躯分布特征及对3种杀虫剂敏感性比较  

Tissue Distribution of Two Detoxifying Enzymes in Dendrolimus superans and Their Susceptibility to Three Pesticides

作　　者：邹传山[1] 曹传旺[1] 张国财[1] 王志英[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《林业科学》2013年第3期165-169,共5页Scientia Silvae Sinicae

基　　金：国家林业公益性行业科研专项课题(201104069);黑龙江省科技计划项目(GB08B202-02;GA09B203-3);中央高校基本科研业务费专项资金项目(DL11BA17)

摘　　要：落叶松毛虫（Dendrolimussuperans）又称西伯利亚松毛虫，隶属鳞翅目（Lepidoptera）枯叶蛾科（Lasiocampidae）。国内分布于北京、东北、内蒙古、新疆北部，是我国东北林区的重要害虫，除危害落叶松（Larixspp．）外，也危害红松（Pinuskoraiensis）、油松（P．tabulaeformis）、樟子松（P．sylvestrisvar．mongolica）、云杉（Piceaspp．）、冷杉（Abiesspp．）等主要北方针叶树种。Distribution of carboxylesterase （CarE） and glutathione-S transferase （GST） in the midgut, fat body, head and epidemis tissues of Dendrolimus superans 6＇h instar was determined. The results showed that both CarE and GST distributed in all the four tested tissues, however, CarE predominated in midgut （61.36%） while GST in fat body （36. 15% ）. The susceptibility of CarE and GST to DDVP, avermectins and lambda-cyhalothrin was also measured in vitro. The three pesticides had different degree inhibition to either CarE or GST. There were distinct dose-effect relationships between each pesticide and each enzyme. DDVP showed the highest inhibitive effect to Care （IC50 〈 1. 000 × 10-4 mg·mL-1 ）, followed by avermectins （IC50 = 1. 259 × 10-2mg·mL-1） and then lambda-cyhalothrin （IC50 =4. 529 ×10-1 mg·mL-l）. However, avermectins showed the highest inhibitive effect to GST, followed by lambda-eyhalothrin, DDVP showed the weakest inhibition to GST. The ICsovalues were 8. 044 × 10 -3, 9. 224 × 10 -3 and 1. 755 × 10 -2 mg· mL-1 respectively. These results provide important information for reasonable insecticide application to control D. superans.

关 键 词：落叶松毛虫 羧酸酯酶 谷胱甘肽-S转移酶 杀虫剂 敏感性 

分 类 号：S763.3[农业科学—森林保护学]