通络醒脑泡腾片对Aβ海马注射痴呆大鼠tau蛋白磷酸化关键调控蛋白CDK5和GSK-3表达的影响  

Effects of Tongluo Xingnao effervescent tablet on expression of CKD5 and GSK-3 in rat model of hippocampus with Aβ injection

作　　者：徐世军[1,2] 代渊[2] 张荫杰[1,2] 熊敏[1,2] 马云桐[1,2] 钟振东[3] 

机构地区：[1]四川省中药资源系统研究与开发利用国家重点实验室培育基地,四川成都611137 [2]成都中医药大学,四川成都611137 [3]成都中医药大学附属医院,四川成都611137

出　　处：《华西药学杂志》2013年第2期140-143,共4页West China Journal of Pharmaceutical Sciences

基　　金："重大新药创制"科技重大专项(2013ZX09103002-008);四川省杰出青年学术技术带头人资助计划(2011JQ0014);四川省学术和技术带头人培养资金资助项目(重点);四川省"十一五"支撑项目(2010SZ0195);四川省教育厅自然科学重点项目(12ZA041);成都市产业发展技术支撑计划-高校院所应用基础研究(12DXYB321JH-002)

摘　　要：目的探讨通络醒脑泡腾片对AD大鼠学习记忆和海马CKD5和GSK-3表达的影响,为其防治阿尔茨海默病提供依据。方法建立Aβ25-35海马注射痴呆大鼠模型,采用Morris水迷宫检测大鼠学习记忆能力,采用免疫组织化学和图像分析技术定量分析大鼠海马CKD5和GSK-3表达。结果通络醒脑泡腾片能显著缩短训练第3天和第5天大鼠定向航行测试的逃避潜伏期,显著延长空间探索测试第一象限时间和有效区停留时间,缩短第三象限时间;能够显著降低海马区CDK5和GSK-3的表达,平均光密度值显著降低。结论通络醒脑泡腾片具有提高AD大鼠学习记忆能力,抑制海马CDK5和GSK-3表达的作用,提示其益智作用与抑制海马CDK5和GSK-3有关。OBJECTIVE To provide the basis for preventing and treating Alzheimer＇ s disease by investigating the effects of Tongluo Xingnao effervescent Tablet on learning and memory, hippoeampus CKD5 and GSK - 3 expression in AD rats. METHODS AD rat models of Aβ25-35hippoeampus injection were established, and evaluate learning and memory ability of AD rat were evaluated by the first quadrant dwell time and effective quadrant dwell time,and decreased the third quadrant dwell time in space exploration test signifi- cantly. It also inhibited the expression of hippoeampal CKD5 and GSK - 3. CONCLUSION Tongluo Xingnao effervescent tablet can improve learning and memory of AD rat and inhibit the expression of hippoeampat CKD5 and GSK - 3, suggesting its potential role relat-ed to the suppression of tau hyperphosphorylation.

关 键 词：通络醒脑泡腾片 学习记忆 海马 CDK5 GSK-3 阿尔茨海默病 

分 类 号：R96[医药卫生—药理学]