HPLC与UPLC测定低分子量鱼精蛋白方法的比较  

Comparison of UPLC and HPLC for the determination of low molecular weight protamine

作　　者：尹超[1,2] 盛剑勇[2] 秦晶[2] 李萍[1] 王建新[2] 

机构地区：[1]西南交通大学药学院,四川成都610031 [2]复旦大学药学院,上海201203

出　　处：《华西药学杂志》2013年第2期181-183,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC法与超高压液相色谱(UPLC)法测定低分子量鱼精蛋白(LMWP)的含量。方法分别对两种测定方法的条件进行了优化,建立了测定的方法学。结果 HPLC采用AgelaVenusil ASB C18色谱柱(250 mm×4.6 mm,5μm),流动相为含0.1%三氟乙酸的水-乙腈(14∶86),流速1 mL.min-1,检测波长214 nm,柱温40℃。UPLC采用Acquity UPLC BEHC18色谱柱(50 mm×2.1 mm,1.7μm),流动相为含0.1%三氟乙酸的水-乙腈(1∶9),流速0.4 mL.min-1,检测波长214 nm,柱温40℃。结论 HPLC和UPLC都能用于LMWP的检测,但UPLC的检测时间更短、检测限更低。OBJECTIVE To establish and compare ultra - performance liquid chromatography （ UPLC ） and HPLC methods for the determination of low molecular weight protamine （LMWP）. METHODS The methods of HPLC and UPLC were optimized and used to determine the amount of LMWP. RESULTS For HPLC method, an AgelaVenusi[ ASB Cls （250 mm×4.6 mm, 5 μm） column was applied. Acetonitrile- water（14：86） -0.1% TFA was used as mobile phase. The flow rate and detective wavelength were set as 1 mL·min^-1 and 214 nm, respeetively. For UPLC method, an Acquity UPLC BEH C18 （50mm×2.1 mm, 1.7 μm） column was chosen, and acetonitrile - water（10：90） with 0.1% TFA was used as mobile phase. The flow rate was set at 0.4 mL·min^-1 and the detective wavelength was set at 214 nm. CONCLUSION Both methods are qualified for the determination of LMWP. Compared with HPLC, UPLC is more efficient and sensitive.

关 键 词：低分子量鱼精蛋白 含量测定 超高效液相色谱 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]