橘红胶囊的TLC鉴别及柚皮苷、橙皮苷的含量测定  

Identification of Juhong capsules by TLC and determination of naringin and hesperdin

作　　者：孙韬[1] 包小红[1] 

机构地区：[1]四川省食品药品检验所,四川成都610097

出　　处：《华西药学杂志》2013年第2期186-188,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的完善橘红胶囊的质量标准。方法采用TLC法同板定性鉴别橘红胶囊中的浙贝母、陈皮,采用HPLC法同一色谱条件定量检测柚皮苷、橙皮苷的含量。结果 TLC中分别检出浙贝母、陈皮,斑点清晰可见,重复性好。柚皮苷线性范围为0.0987～1.9740μg(r=0.9999),平均回收率96.51%,RSD=0.88%(n=6);橙皮苷线性范围为0.0987～3.9480μg(r=0.9999),平均回收率98.55%,RSD=0.58%(n=6)。结论所用方法简便、准确、高效、环保,能有效控制橘红胶囊的质量。OBJECTIVE To improve the quality specification of Juhong capsules. METHODS TLC was used to identify bulbus fritillariae thunbergii and pericarpium citri reticulatae in Juhong capsules. HPLC was used to determine the content of naringin and hesperidin. RESULTS Bulbus fritillariae thunbergii and pcricarpium citri rcticulatae in Juhong capsules were found on TLC. The spots were clear. The method showed good reproducibility. The linear range of naringin was in a range of 0.0987 - 1.9740 μg（r = 0.9999）. The average recovery rate of naringin was 96.51% with a RSD of 0.88% （n = 6）. The linear range of hesperdin was in a range of 0.0987 - 3.9480 μg（r = 0.9999）. The average recovery rate of hesperdin was 98.5 % with a RSD of 0.58% （n = 6）. CONCLUSION The method is simple, specific, sensitive,efficient,eco- friendly and can be applied to quality control of Juhong capsules.

关 键 词：橘红胶囊 薄层色谱法 高效液相色谱法 柚皮苷 橙皮苷 

分 类 号：R917[医药卫生—药物分析学]