HPLC测定不同产地白术水煎液中的白术内酯Ⅲ  

Determination of atractylode Ⅲ in the water solution of Atractylodis macrocephalae Rhizoma from different habitats by HPLC

作　　者：李鸿翔[1] 彭腾[1] 邓赟[1] 李柏群[2] 杨菁[1] 黎豫川[1] 范静骞[1] 

机构地区：[1]成都中医药大学药学院教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都610075 [2]重庆三峡中心医院药剂科,重庆万州404000

出　　处：《华西药学杂志》2013年第2期197-198,共2页West China Journal of Pharmaceutical Sciences

基　　金：四川省教育厅重点课题(编号:09ZA028;编号:10ZA095);重庆市科委重点项目(编号:CSTC2010AC5095);成都市科技局科技攻关项目(编号:10GGYB303SW-023);成都中医药大学校基金重点课题(编号:ZRZD201101)

摘　　要：目的测定不同产地白术水煎液中白术内酯Ⅲ的含量。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(55∶45),检测波长220 nm,流速1 mL.min-1,柱温30℃。结果白术内酯Ⅲ在测定范围内线性关系良好,平均回收率为97.00%,RSD=1.04%。结论所用方法操作简单、快捷、重复性好、准确度高,可用于白术的质量控制。OBJECTIVE To determine the content of atractylode Ⅲ in water solution of Atractylodis macrocephalae Rhizoma from different habitats. METHODS The HPLC separation was detected with DAD at 220 nm and was carried out with methyl cyanides - water（55：45） as the mobile phase at a flow rate of 1 mL·min^-1. The column temperature was 30℃. RESULTS The linear scope was good in the range of determination, the average recovery was 97.00% , and the relative standard deviations （RSD） was 1.04%. CONCLUSION The method is simple, fast, well repeaetable and highly accurate, high accuracy, which can be used as the quality control method for Atractylodis macrocephalae Rhizoma.

关 键 词：高效液相色谱法 不同产地 白术 白术内酯Ⅲ 含量测定 

分 类 号：R917[医药卫生—药物分析学]