老鸭嘴化学成分研究  

Chemical constituents in Thunbergia from Africa

作　　者：黄凤杰[1] 宋建晓[1] 刘佳健[2] 赵爱华[1,2] 贾伟[2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海交通大学第六附属人民医院转化医学中心,上海200233

出　　处：《中国中药杂志》2013年第8期1183-1187,共5页China Journal of Chinese Materia Medica

摘　　要：目的:对老鸭嘴乙醇提取物的活性部位进行化学成分研究。方法:对老鸭嘴提取液不同极性部位进行醛糖还原酶抑制活性筛选,活性部位应用常规柱色谱及制备高效液相手段相结合进行化学成分分离,采用波谱技术对所得到的化合物进行结构鉴定。结果:从乙酸乙酯部位中分离鉴定了8个化合物,分别为新化合物8-羟基-8-甲基-9-亚甲基-环戊烷[7,11]-1,4,6-三羟基-四氢化萘-12-酮,命名为山牵牛素A(1),3,4-二氢-4,5,8-三羟基-2-(3-甲基-2-丁烯基)萘[2,3-b]环氧乙烯-1(2H)-酮(2),8-(β-葡萄糖吡喃型-O-)-3,4-二氢-2-(3-甲基-2-丁烯基)萘[2,3-b]环氧乙烯-1(2H)-酮(3),高良姜素(4),槲皮素(5),木犀草素(6),5,6,3',4'-四羟基-3,7-二甲氧基黄酮(7)和羽扇豆醇(8)。结论:老鸭嘴乙酸乙酯提取物有明显的抑制醛糖还原酶活性,从该提取物中分离得到1个新的四氢化萘衍生物山牵牛素A,首次从该属植物中分离得到环氧萘类化合物及其苷,还有4个黄酮类化合物,1个三萜化合物。Objective： To investigate the chemical compounds from the ethanol extract with inhibitory effects against aldose re- ductase from Thunbergia. Method： Guided by anti-aldose reductase assay, compounds from the bioactive fraction （ethyl acetate ex- tract） were separated and purified by various chromatographic methods including silica gel, Sephadex LH-20, and reversed-phase HPLC. Their structures were indentified based on analysis of the spectroscopic data including 1D and 2D NMR data. Result： Eight compounds were obtained and identified as 8-hydroxy-8-methyl-9-methene-cyclopentane [ 7,11 ] -1,4,6-trihydroxy-tetrahydronaphtha- lene-12-one, named as thunbergia A （ 1 ）, 3,4-dihydro-4,5,8-trihydroxy-2- （ 3-methyl-2-butenyl ） naphtha [ 2,3-b ] oxiren-1 （ 2H ） -one （ 2 ）, 8- （β-gluco pyranosyloxy） -3,4 -dihydro-2 - （ 3-methyl-2 -butenyl ） naphtha [ 2,3-b ] oxiren-1 （ 2 H） -one （ 3 ）, galangin （ 4 ）, quer- cetin （5）, luteolin （6）, 5,6,3＇,4＇-tetrahydroxy-3,7-dimethoxy-flavone （7） and upeol （8）. Conclusion： Thunbergia A was a new derivative of tetrahydronaphthalene, and compounds 2 and 3 were separated from the genus Thunbergia for the first time.

关 键 词：爵床科 山牵牛属 老鸭嘴 醛糖还原酶活性 山牵牛素A 

分 类 号：R284[医药卫生—中药学]